Sterzl I, Hampl R, Sterzl J, Votruba J, Stárka L
Institute of Endocrinology, Praha, Czech Republic.
J Steroid Biochem Mol Biol. 1999 Dec 15;71(3-4):133-7. doi: 10.1016/s0960-0760(99)00134-x.
The effect of dexamethasone and of three potential antiglucocorticoids, namely dehydroepiandrosterone (DHEA) and its 7alpha-and 7beta-hydroxylated metabolites, on primary immune response has been studied by measuring the number of plaque forming cells (NPFC) and their viability in a cell culture of murine spleenocytes. As expected, dexamethasone suppressed considerably the NPFC as well as their viability. Surprisingly, DHEA as well as its 7alpha-hydroxylated metabolite decreased significantly the NPFC, while the effect of 7beta-hydroxy-DHEA was different: at low doses it decreased the NPFC, but this effect was less pronounced at higher concentrations. In addition, 7beta-hydroxy-DHEA was able to counteract the effect of dexamethasone on the NPFC. None of the natural steroids affected the cell viability.
通过检测小鼠脾细胞培养物中噬斑形成细胞（NPFC）的数量及其活力，研究了地塞米松以及三种潜在的抗糖皮质激素，即脱氢表雄酮（DHEA）及其7α-和7β-羟基化代谢产物对初次免疫反应的影响。正如预期的那样，地塞米松显著抑制了NPFC及其活力。令人惊讶的是，DHEA及其7α-羟基化代谢产物显著降低了NPFC，而7β-羟基-DHEA的作用则不同：低剂量时它降低了NPFC，但在较高浓度下这种作用不太明显。此外，7β-羟基-DHEA能够抵消地塞米松对NPFC的作用。这些天然类固醇均未影响细胞活力。
