Hiraga S, Ito H, Yamakawa H, Ohtsubo N, Seo S, Mitsuhara I, Matsui H, Honma M, Ohashi Y
Department of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Mol Plant Microbe Interact. 2000 Feb;13(2):210-6. doi: 10.1094/MPMI.2000.13.2.210.
In Tobacco mosaic virus (TMV)-infected tobacco plants carrying the N resistance gene, a hypersensitive reaction or response (HR) occurs to enclose the virus in the infected tissue. Although a contribution of peroxidases to the resistance has been proposed, no evidence has been presented that tobacco peroxidase genes respond to HR. Here, we describe the HR-induced expression of a tobacco peroxidase gene (tpoxC1) whose induction kinetics were slightly different from those of acidic and basic tobacco pathogenesis-related (PR) protein genes. Interestingly, tpoxC1 was insensitive to the inducers of PR genes such as salicylic acid, methyl jasmonate, and ethephon. Spermine activated tpoxC1 gene expression at a low level and both acidic and basic PR gene expression at a considerably higher level. These results indicate that the induced expression of tpoxC1 is regulated differently from that of classical tobacco PR genes in the N gene-mediated self-defense system in tobacco plants.
在携带N抗性基因的烟草花叶病毒（TMV）感染的烟草植株中，会发生过敏反应或应答（HR），将病毒封闭在受感染组织中。尽管有人提出过氧化物酶对这种抗性有贡献，但尚无证据表明烟草过氧化物酶基因会对HR作出反应。在此，我们描述了一个烟草过氧化物酶基因（tpoxC1）的HR诱导表达，其诱导动力学与酸性和碱性烟草病程相关（PR）蛋白基因的诱导动力学略有不同。有趣的是，tpoxC1对PR基因的诱导剂如水杨酸、茉莉酸甲酯和乙烯利不敏感。精胺在低水平激活tpoxC1基因表达，而在相当高的水平激活酸性和碱性PR基因表达。这些结果表明，在烟草植株的N基因介导的自我防御系统中，tpoxC1的诱导表达与经典烟草PR基因的诱导表达调控方式不同。
