Kumar Dilip, Rampuria Sakshi, Singh Naveen Kumar, Kirti Pulugurtha B
Department of Plant Sciences School of Life Sciences University of Hyderabad India.
FEBS Open Bio. 2016 Feb 25;6(3):200-10. doi: 10.1002/2211-5463.12040. eCollection 2016 Mar.
A novel zinc-binding alcohol dehydrogenase 2 (AdZADH2) was significantly upregulated in a wild peanut, Arachis diogoi treated with conidia of late leaf spot (LLS) pathogen, Phaeoisariopsis personata. This upregulation was not observed in a comparative analysis of cultivated peanut, which is highly susceptible to LLS. This zinc-binding alcohol dehydrogenase possessed a Rossmann fold containing NADB domain in addition to the MDR domain present in all previously characterized plant ADH genes/proteins. Transient over-expression of AdZADH2 under an estradiol inducible promoter (XVE) resulted in hypersensitive response (HR)-like cell death in tobacco leaf. However, the same level of cell death was not observed when the domains were transiently expressed individually. Cell death observed in tobacco was associated with overexpression of cell death related proteins, antioxidative enzymes such as SOD, CAT and APX and pathogenesis-related (PR) proteins. In A. diogoi, AdZADH2 expression was significantly upregulated in response to the plant signaling hormones salicylic acid, methyl jasmonate, and sodium nitroprusside.
一种新型锌结合醇脱氢酶2（AdZADH2）在野生花生Arachis diogoi中显著上调，该野生花生用晚叶斑病（LLS）病原体Phaeoisariopsis personata的分生孢子处理。在对高度易感LLS的栽培花生的比较分析中未观察到这种上调。这种锌结合醇脱氢酶除了具有所有先前表征的植物ADH基因/蛋白质中存在的MDR结构域外，还拥有一个包含NADB结构域的Rossmann折叠。在雌二醇诱导型启动子（XVE）下AdZADH2的瞬时过表达导致烟草叶片出现类似过敏反应（HR）的细胞死亡。然而，当这些结构域单独瞬时表达时，未观察到相同程度的细胞死亡。在烟草中观察到的细胞死亡与细胞死亡相关蛋白、抗氧化酶如超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和抗坏血酸过氧化物酶（APX）以及病程相关（PR）蛋白的过表达有关。在A. diogoi中，AdZADH2的表达在响应植物信号激素水杨酸、茉莉酸甲酯和硝普钠时显著上调。
