MFASAT：一种从猕猴（猕猴科，灵长目）中分离出的新型α卫星DNA序列。

MFASAT: a new alphoid DNA sequence isolated from Macaca fascicularis (Cercopithecidae, Primates).

作者信息

Crovella S, Del Pero M, Marziliano N, Garagna S, Pecile V, Morgutti M, Boniotto M, Amoroso A, Montagnon D, Campa C C, Lamberti L, Bigatti M P, Ardito G

机构信息

Cattedra di Genetica e Servizio di Genetica, IRCCS Burlo-Garofolo, Trieste, Italy.

出版信息

Genome. 1999 Dec;42(6):1066-70. doi: 10.1139/g99-054.

DOI:10.1139/g99-054

PMID:10659771

Abstract

A new highly repeated DNA fragment isolated from Macaca fascicularis (MFASAT) is described. Our findings obtained by sequencing, Southern blot analysis, and fluorescent in situ hybridization (FISH) on metaphasic chromosomes strongly suggest that MFASAT can be considered as a member of the alphoid DNA family characteristic of Old World monkeys. The chromosomal localization of MFASAT, obtained by FISH, showed that this alphoid DNA is present in the peri-centromeric area of all the chromosomes. MFASAT showed a high degree of conservation when compared, by sequence alignment, to other Macaca species and Papio papio as expected for species with considerable genome conservation. A low degree of homology has been found comparing M. fascicularis alphoid DNA with a more distantly related Cercopithecidae species such as Cercopithecus aethiops.

摘要

本文描述了从食蟹猴（Macaca fascicularis）中分离出的一个新的高度重复DNA片段（MFASAT）。我们通过对中期染色体进行测序、Southern印迹分析和荧光原位杂交（FISH）获得的结果强烈表明，MFASAT可被视为旧世界猴特有的α卫星DNA家族的成员。通过FISH获得的MFASAT的染色体定位表明，这种α卫星DNA存在于所有染色体的着丝粒周围区域。通过序列比对，与其他猕猴属物种和狒狒（Papio papio）相比，MFASAT显示出高度的保守性，这对于具有相当基因组保守性的物种来说是预期的。将食蟹猴α卫星DNA与亲缘关系更远的猕猴科物种如黑猩猩（Cercopithecus aethiops）进行比较时，发现同源性较低。