一种植物防御蛋白——菜豆中的多聚半乳糖醛酸酶抑制蛋白的初步X射线晶体学分析。
Preliminary X-ray crystallographic analysis of a plant defence protein, the polygalacturonase-inhibiting protein from Phaseolus vulgaris.
作者信息
Leech A, Mattei B, Federici L, De Lorenzo G, Hemmings A M
机构信息
School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England.
出版信息
Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):98-100. doi: 10.1107/s0907444999014316.
A leucine-rich repeat plant protein involved in resistance to pathogens, a polygalacturonase-inhibiting protein (PGIP-1) from Phaseolus vulgaris, has been crystallized and preliminary X-ray characterization has been performed. The protein contains ten repeats of a short (24 amino-acid) leucine-rich repeat motif. Single crystals of the protein were grown from vapour-diffusion experiments using PEG 2K monomethylether as precipitant; these crystals diffract to at least 2.3 A resolution. The space group is P2(1), with two molecules of PGIP-1 in the asymmetric unit; the crystals contain approximately 38% solvent.
一种参与病原体抗性的富含亮氨酸重复序列的植物蛋白，即来自菜豆的多聚半乳糖醛酸酶抑制蛋白（PGIP-1），已被结晶，并进行了初步的X射线表征。该蛋白包含十个短（24个氨基酸）富含亮氨酸重复基序的重复序列。使用聚乙二醇2K单甲醚作为沉淀剂，通过气相扩散实验生长出该蛋白的单晶；这些晶体的衍射分辨率至少为2.3埃。空间群为P2(1)，不对称单元中有两个PGIP-1分子；晶体含有约38%的溶剂。
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