Peraçoli M T, Montelli T C, Soares A M, Parise-Fortes M R, Alquati S A, Ueda A, Montenegro M R, Alves A, Gabarra R C, Faleiros T P, Zanini M A
Department of Microbiology and Immunology, State University of São Paulo (UNESP), Botucatu, Brazil.
Arq Neuropsiquiatr. 1999 Sep;57(3A):539-46. doi: 10.1590/s0004-282x1999000400001.
Natural killer (NK) cells play an important role in immune surveillance against tumors. The present work aimed to study the cytotoxic activity of NK cells and T cell subsets in peripheral blood of 13 patients with primary tumors in central nervous system (CNS). As controls 29 healthy subjects with the age range equivalent to the patients were studied. The methods employed were: a) determination of cytotoxic activity of NK cells towards K562 target cells, evaluated by single cell-assay; b) enumeration of CD3+ lymphocytes and their CD4+ and CD8+ subsets defined by monoclonal antibodies; c) the identification of tumors were done by histologic and immunochemistry studies. The results indicated that adults and children with tumor in CNS display reduced percentage of total T cells, helper/inducer subset and low helper/suppressor ratio. The cytotoxic activity of NK cells was decreased in patients with CNS tumors due mainly to a decrease in the proportion of target-binding lymphocytes. These results suggest that cytotoxic activity of NK cells may be affected by the immunoregulatory disturbances observed in patients with primary tumors in CNS.
自然杀伤（NK）细胞在针对肿瘤的免疫监视中发挥着重要作用。目前的工作旨在研究13例中枢神经系统（CNS）原发性肿瘤患者外周血中NK细胞和T细胞亚群的细胞毒性活性。作为对照，研究了29名年龄与患者相当的健康受试者。所采用的方法包括：a）通过单细胞测定评估NK细胞对K562靶细胞的细胞毒性活性；b）用单克隆抗体对CD3 +淋巴细胞及其CD4 +和CD8 +亚群进行计数；c）通过组织学和免疫化学研究对肿瘤进行鉴定。结果表明，患有CNS肿瘤的成人和儿童显示总T细胞、辅助/诱导亚群的百分比降低，且辅助/抑制比例较低。CNS肿瘤患者的NK细胞细胞毒性活性降低，主要是由于靶细胞结合淋巴细胞比例下降。这些结果表明，NK细胞的细胞毒性活性可能受到CNS原发性肿瘤患者中观察到的免疫调节紊乱的影响。
