Axt R, Kordina A C, Meyberg R, Reitnauer K, Mink D, Schmidt W
Department of Obstetrics and Gynecology, University of the Saarland, Homburg, Germany.
Clin Exp Obstet Gynecol. 1999;26(3-4):195-8.
To investigate the extent of apoptosis within the human placenta in tissues from normotensive term pregnancies and those complicated by intrauterine growth-restriction (IUGR).
A total of 18 placentas, 10 obtained from uncomplicated term gestations and 8 from intrauterine growth restricted fetuses were included in this study. Apoptosis was identified using a terminal deoxynucleotidyl transferase-mediated deoxyuridine triophosphate nick end-labeling technique (TUNEL, Boehringer, Mannheim, Germany) in paraffin-embedded sections.
Apoptosis was predominantly detected in the villous trophoblast and stromal tissue. There were no differences in the incidence of apoptosis in different parts of the same placenta. The apoptotic index in placental tissue from uncomplicated pregnancies was 0.93 +/- 0.12. Significantly more apoptotic nuclei were detected in the placental tissue from IUGR gestation (4.2 +/- 2.96, p < 0.01).
These results might point toward a possible role of apoptosis in the pathophysiology of intrauterine growth-restriction.
研究正常血压足月妊娠组织及合并宫内生长受限（IUGR）的妊娠组织中人胎盘的凋亡程度。
本研究共纳入18个胎盘，其中10个来自无并发症的足月妊娠，8个来自宫内生长受限胎儿。采用末端脱氧核苷酸转移酶介导的脱氧尿苷三磷酸缺口末端标记技术（TUNEL，德国曼海姆宝灵曼公司）在石蜡包埋切片中鉴定凋亡。
凋亡主要在绒毛滋养层和间质组织中检测到。同一胎盘不同部位的凋亡发生率无差异。无并发症妊娠胎盘组织的凋亡指数为0.93±0.12。在IUGR妊娠的胎盘组织中检测到的凋亡核明显更多（4.2±2.96，p<0.01）。
这些结果可能表明凋亡在宫内生长受限的病理生理学中可能起作用。
