气相色谱-串联质谱法同时测定小麦（普通小麦）中的酚酸和2,4-二羟基-7-甲氧基-1,4-苯并恶嗪-3-酮

Simultaneous determination of phenolic acids and 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one in wheat (Triticum aestivum L.) by gas chromatography-tandem mass spectrometry.

作者信息

Wu H, Haig E, Pratley J, Lemerle D, An M

机构信息

Farrer Centre for Conservation Farming, Charles Sturt University, Wagga Wagga, NSW, Australia.

出版信息

J Chromatogr A. 1999 Dec 24;864(2):315-21. doi: 10.1016/s0021-9673(99)01034-1.

DOI:10.1016/s0021-9673(99)01034-1

PMID:10669299

Abstract

A procedure using gas chromatography and tandem mass spectrometry (GC-MS-MS) has been developed for the identification and quantification of some allelochemicals in wheat (Triticum aestivum L.). The quantities of allelochemicals in wheat shoots ranged from 2.9 to 110 mg per kilogram of dry shoot residues. Compared with gas chromatography-mass spectrometry (GC-MS), the GC-MS-MS technique significantly increased instrument selectivity and sensitivity, thereby providing more reliable quantitation results in the determination of the phytotoxic compounds examined during this allelopathy research.

摘要

已开发出一种使用气相色谱-串联质谱法（GC-MS-MS）的程序，用于鉴定和定量小麦（普通小麦）中的一些化感物质。小麦地上部分化感物质的含量范围为每千克干地上部分残体2.9至110毫克。与气相色谱-质谱法（GC-MS）相比，GC-MS-MS技术显著提高了仪器的选择性和灵敏度，从而在本次化感作用研究中所检测的植物毒性化合物的测定中提供了更可靠的定量结果。