van Dongen M J, Cederberg A, Carlsson P, Enerbäck S, Wikström M
Department of Structural Chemistry, Pharmacia and Upjohn, Nordenflychtsvägen 62:5, Stockholm, S-11287, Sweden.
J Mol Biol. 2000 Feb 18;296(2):351-9. doi: 10.1006/jmbi.1999.3476.
Transcription factors of the forkhead type share a highly conserved DNA-binding domain of about 100 amino acid residues. FREAC-11, expressed in adipocytes, belongs to this class. Here, we report on NMR studies that established the three-dimensional structure of the FREAC-11, DNA-binding domain. Although apparent similarities to the structures of other members within the forkhead family are observed, the structure also reveals some remarkable differences. Along with the complementary dynamics, the data provide insight into the fundamentals of sequence specificity within a highly conserved motif.
叉头型转录因子具有一个由约100个氨基酸残基组成的高度保守的DNA结合结构域。在脂肪细胞中表达的FREAC-11属于这一类。在此，我们报告了确定FREAC-11 DNA结合结构域三维结构的核磁共振研究。虽然观察到与叉头家族其他成员的结构有明显相似之处，但该结构也显示出一些显著差异。连同互补动力学一起，这些数据为深入了解一个高度保守基序内序列特异性的基本原理提供了线索。
