Mulholland D J, Wilson J, Vogl W, Webber W
Department of Anatomy, Faculty of Medicine, Vancouver, Canada.
Tissue Cell. 1999 Dec;31(6):610-6. doi: 10.1054/tice.1999.0075.
In this study we define the distribution of actin bundle arrangement in Bowman's capsule of rat renal corpuscles. Parietal cells of Bowman's capsule were examined by conventional light microscopy, electron microscopy and confocal microscopy. Within each parietal cell individual actin bundles are arranged in a parallel fashion running the length of the cell. Computer reconstructions obtained using confocal microscopy clearly show the lengths of actin bundles to be arranged, on a capsule level, end-to-end, at angles and perpendicular to bundles in adjacent cells. The bundles stain positively for non-muscle myosin and vinculin. The presence and arrangement of actin bundles in parietal cells is consistent with a role in reinforcing capsule structure.
在本研究中，我们定义了大鼠肾小体鲍曼囊内肌动蛋白束排列的分布情况。通过传统光学显微镜、电子显微镜和共聚焦显微镜对鲍曼囊的壁层细胞进行了检查。在每个壁层细胞内，单个肌动蛋白束以平行方式排列，贯穿细胞全长。使用共聚焦显微镜获得的计算机重建图像清楚地显示，在囊水平上，肌动蛋白束的长度呈端对端、成角度以及与相邻细胞中的束垂直排列。这些束对非肌肉肌球蛋白和纽蛋白呈阳性染色。壁层细胞中肌动蛋白束的存在和排列与增强囊结构的作用一致。
