识别福尔马林固定、石蜡包埋组织中CD5、CD10和CD23的单克隆抗体：其制备及在小B细胞淋巴瘤诊断中的价值评估

Monoclonal antibodies recognizing CD5, CD10 and CD23 in formalin-fixed, paraffin-embedded tissue: production and assessment of their value in the diagnosis of small B-cell lymphoma.

作者信息

Watson P, Wood K M, Lodge A, McIntosh G G, Milton I, Piggott N H, Proctor S J, Taylor P R, Smith S, Jack F, Bell H, Steward M, Anderson J J, Horne C H, Angus B

机构信息

Department of Pathology and Haematology, The University of Newcastle upon Tyne, and Novocastra Laboratories Ltd., Newcastle-upon-Tyne, UK.

出版信息

Histopathology. 2000 Feb;36(2):145-50. doi: 10.1046/j.1365-2559.2000.00813.x.

DOI:10.1046/j.1365-2559.2000.00813.x

PMID:10672059

Abstract

AIMS

Assessment of the expression of antigens CD5, CD10 and CD23 can be of value in the differential diagnosis of small B-cell lymphoma. Correct subclassification is important since optimal treatment regimes differ between the subtypes. The aim of this study was to generate monoclonal antibodies recognizing these antigens in paraffin-embedded tissue and to assess their efficacy using a panel of cases of small B-cell lymphoma of various subtypes.

METHODS AND RESULTS

For each antibody synthetic recombinant protein and conventional murine hybridoma technology was employed. Monoclonal antibodies effective in formalin-fixed, paraffin-embedded tissue were successfully generated, designated NCL-CD5-4C7, NCL-CD10-270 and NCL-CD23-1B12, respectively. A series of 58 cases of small B-cell lymphoma including examples of each subtype (lymphocytic, follicle centre cell, mantle cell, marginal zone and lymphoplasmacytoid) was assembled and immunostaining for the respective antigens carried out using the monoclonal antibodies produced. Our results indicate that the antibodies are specific for their respective antigens and give the predicted phenotypic profile in the small B-cell lymphoma subtypes.

CONCLUSIONS

These novel monoclonal antibodies may be of value in routine diagnostic practice.

摘要

目的

评估抗原CD5、CD10和CD23的表达在小B细胞淋巴瘤的鉴别诊断中可能具有价值。正确的亚分类很重要，因为不同亚型的最佳治疗方案有所不同。本研究的目的是制备能够识别石蜡包埋组织中这些抗原的单克隆抗体，并使用一组不同亚型的小B细胞淋巴瘤病例评估其有效性。

方法与结果

针对每种抗体，采用了合成重组蛋白和传统的小鼠杂交瘤技术。成功制备了在福尔马林固定、石蜡包埋组织中有效的单克隆抗体，分别命名为NCL-CD5-4C7、NCL-CD10-270和NCL-CD23-1B12。收集了一系列58例小B细胞淋巴瘤病例，包括各亚型（淋巴细胞型、滤泡中心细胞型、套细胞型、边缘区型和淋巴浆细胞型）的样本，并使用所制备的单克隆抗体对相应抗原进行免疫染色。我们的结果表明，这些抗体对各自的抗原具有特异性，并在小B细胞淋巴瘤亚型中呈现出预期的表型特征。