de Leon E D, Alkan S, Huang J C, Hsi E D
Department of Pathology, Loyola University Medical Center, Maywood, Illinois, USA.
Mod Pathol. 1998 Nov;11(11):1046-51.
Diagnosis of small B-cell lymphomas is sometimes difficult without fresh tissue for flow cytometry (FC) or immunohistochemistry (IHC). Therefore, we examined the usefulness of a paraffin section IHC panel consisting of antibodies to CD5, CD10, CD20, CD23, CD43, and cyclin D1. We tested 55 formalin-fixed small B-cell lymphomas, including 16 small lymphocytic lymphomas (SLLs), 10 mantle cell lymphomas (MCLs), 25 follicle center lymphomas (FCLs), and 4 mantle zone lymphomas (MZLs). Seventeen cases had B5-fixed sections that were stained in the same manner. The findings were correlated with FC immunophenotyping when available. All of the SLLs and 90% of the MCLs expressed CD5 by IHC, with occasional weak expression in some MCLs. All of the FCLs and MZLs lacked CD5 expression. These results were comparable to those obtained by FC. CD43 expression was seen in 100% of the SLLs, 90% of the MCLs, and 75% of the MZLs. CD23 expression was seen in 94% of the SLL; of these, 100% also showed expression of CD23 by FC. Cyclin D1 was detected in all of the MCLs by IHC but also in 3 of the 16 SLLs. CD23 was absent in all of the MCLs. CD10 expression was present in 21 (95%) of 22 FCLs. All of the 17 cases fixed in B5 showed a decreased immunoreactivity for CD5 in the neoplastic cells. In contrast, CD10 immunoreactivity was judged better in B5-fixed sections. We concluded, therefore, that anti-CD5 and -CD10 were useful tools in the differential diagnosis of B-cell lymphomas of small lymphocytes and that a paraffin-section IHC panel consisting of antibodies to CD5, CD10, CD20, CD23, CD43, and cyclin D1 was a useful ancillary technique that compared favorably with FC.
如果没有新鲜组织用于流式细胞术（FC）或免疫组织化学（IHC），小B细胞淋巴瘤的诊断有时会很困难。因此，我们检测了由抗CD5、CD10、CD20、CD23、CD43和细胞周期蛋白D1抗体组成的石蜡切片免疫组织化学检测组合的实用性。我们检测了55例福尔马林固定的小B细胞淋巴瘤，包括16例小淋巴细胞淋巴瘤（SLL）、10例套细胞淋巴瘤（MCL）、25例滤泡中心淋巴瘤（FCL）和4例套区淋巴瘤（MZL）。17例病例有以相同方式染色的B5固定切片。如有可用，将结果与FC免疫表型分析结果进行关联。所有SLL和90%的MCL通过免疫组织化学检测表达CD5，一些MCL偶尔有弱表达。所有FCL和MZL均无CD5表达。这些结果与通过FC获得的结果相当。100%的SLL、90%的MCL和75%的MZL可见CD43表达。94%的SLL可见CD23表达；其中，100%的病例通过FC也显示CD23表达。通过免疫组织化学检测，所有MCL均检测到细胞周期蛋白D1，但16例SLL中的3例也检测到。所有MCL均无CD23表达。22例FCL中的21例（95%）存在CD10表达。17例B5固定的病例中，肿瘤细胞中CD5的免疫反应性均降低。相比之下，B5固定切片中CD10的免疫反应性被判定更好。因此，我们得出结论，抗CD5和抗CD10是鉴别小淋巴细胞B细胞淋巴瘤的有用工具，由抗CD5、CD10、CD20、CD23、CD43和细胞周期蛋白D1抗体组成的石蜡切片免疫组织化学检测组合是一种有用的辅助技术，与FC相比具有优势。
