Chary V K, Hilbert D W, Higgins M L, Piggot P J
Department of Microbiology and Immunology, Temple University School of Medicine, 3400 N. Broad Street, Philadelphia, PA 19140, USA.
Mol Microbiol. 2000 Feb;35(3):612-22. doi: 10.1046/j.1365-2958.2000.01731.x.
The spoIIIE gene of Sporosarcina ureae encodes a 780-residue protein, showing 58% identity to the SpoIIIE protein of Bacillus subtilis, which is thought to be a DNA translocase. Expression of the S. ureae spoIIIE gene is able to restore sporulation in a B. subtilis spoIIIE mutant. Inactivation of the S. ureae spoIIIE gene blocks sporulation of S. ureae at stage III. Within the limits of detection, the sporulation division in S. ureae shows the same symmetry, or near symmetry, as the vegetative division (in contrast to the highly asymmetric location of the sporulation division for B. subtilis), and so it is inferred that SpoIIIE facilitates chromosome partitioning during sporulation, even when the division is not grossly asymmetric. It is suggested that chromosome partitioning lags behind division during sporulation but not during vegetative growth.
尿素芽孢八叠球菌的spoIIIE基因编码一种由780个氨基酸残基组成的蛋白质，与枯草芽孢杆菌的SpoIIIE蛋白有58%的同源性，后者被认为是一种DNA转位酶。尿素芽孢八叠球菌spoIIIE基因的表达能够恢复枯草芽孢杆菌spoIIIE突变体的芽孢形成。尿素芽孢八叠球菌spoIIIE基因的失活会在III期阻断尿素芽孢八叠球菌的芽孢形成。在检测范围内，尿素芽孢八叠球菌的芽孢形成分裂与营养细胞分裂表现出相同的对称性或接近对称性（与枯草芽孢杆菌芽孢形成分裂的高度不对称位置相反），因此可以推断，即使分裂并非严重不对称，SpoIIIE也有助于芽孢形成过程中的染色体分配。有人提出，在芽孢形成过程中染色体分配滞后于细胞分裂，但在营养生长过程中并非如此。
