Nawrot C F, Mittelstadt J K, Kuo M
J Dent Res. 1976 Sep-Oct;55(5):841-7. doi: 10.1177/00220345760550052101.
Denatured collagen samples extracted from the skin and bone of lathyritic rats with guanidine hydrochloride were chromatographed on hydroxyapatite columns. A major fraction of the bone extract that was rich in alpha chain showed a stronger binding to hydroxyapatite when compared to the corresponding fraction from skin. Thus, bone collagen shows a special affinity for hydroxyapatite after isolation and denaturation.
用盐酸胍从患骨软化症大鼠的皮肤和骨骼中提取的变性胶原蛋白样品在羟基磷灰石柱上进行色谱分析。与来自皮肤的相应部分相比，富含α链的骨骼提取物的主要部分对羟基磷灰石表现出更强的结合力。因此，骨胶原蛋白在分离和变性后对羟基磷灰石表现出特殊的亲和力。
