以去-Nα1-乙酰-α-促黑素为底物的蛋白质氨基末端酶促乙酰化的模型研究。
Model studies of enzymatic NH2-terminal acetylation of porteins with des-Nalpha1-acetyl-alpha-melanotropin as a substrate.
作者信息
Granger M, Tesser G I, De Jong W W, Bloemendal H
出版信息
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3010-4. doi: 10.1073/pnas.73.9.3010.
Abstract
The present study describes the acetylation by an enzyme present in calf lens of a synthetic tridecapeptide [analogous to alpha-melanotropin (alpha-melanocyte stimulating hormone) but lacking the naturally occurring NH2-terminal acetyl group: des-Nalpha1-Ac-alpha-melanotropin]. The reaction is specific for the alpha-amino group of the NH2-terminal amino acid. The minimum length required for the substrate to become acetylated appears to be a sequence of five to eight amino acid residues. Modification of the internal lysine decreases the incorporation of acetate, irrespective of the size of the blocking group.
摘要
本研究描述了小牛晶状体中存在的一种酶对一种合成十三肽[类似于α-促黑素（α-黑素细胞刺激激素），但缺乏天然存在的NH2-末端乙酰基：去-Nα1-Ac-α-促黑素]的乙酰化作用。该反应对NH2-末端氨基酸的α-氨基具有特异性。底物被乙酰化所需的最短长度似乎是五到八个氨基酸残基的序列。内部赖氨酸的修饰会降低乙酸盐的掺入，而与封闭基团的大小无关。
相似文献
1
Model studies of enzymatic NH2-terminal acetylation of porteins with des-Nalpha1-acetyl-alpha-melanotropin as a substrate.以去-Nα1-乙酰-α-促黑素为底物的蛋白质氨基末端酶促乙酰化的模型研究。
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3010-4. doi: 10.1073/pnas.73.9.3010.
2
Des-Nalpha-acetyl-alpha-melanotropin: a synthetic substrate for specific N-terminal directed enzymatic acetylation.
Int J Pept Protein Res. 1977 Jan;9(1):52-6.
3
N-acetylation regulates the behavioral activity of alpha-melanotropin in a multineurotransmitter neuron.N-乙酰化作用在多神经递质神经元中调节α-促黑素细胞激素的行为活性。
Science. 1982 Feb 26;215(4536):1125-7. doi: 10.1126/science.7063845.
4
Acetylation of melanocyte-stimulating hormone and beta-endorphin in the pars intermedia of the perinatal pituitary gland in the mouse.小鼠围产期垂体中间部促黑素细胞激素和β-内啡肽的乙酰化作用
Neuroendocrinology. 1986;43(2):166-74. doi: 10.1159/000124524.
5
Mechanism of alpha-melanotropin action.
Bull Schweiz Akad Med Wiss. 1978 Mar;34(1-3):99-111.
6
The bio-organic chemistry of alpha-melanotropin.
Med Res Rev. 1982 Jul-Sep;2(3):247-70. doi: 10.1002/med.2610020303.
7
Synthesis and properties of [11-serine]alpha-melanotropin.
Hoppe Seylers Z Physiol Chem. 1977 Nov;358(11):1475-81. doi: 10.1515/bchm2.1977.358.2.1475.
8
Biological and conformational examination of stereochemical modifications using the template melanotropin peptide, Ac-Nle-c[Asp-His-Phe-Arg-Trp-Ala-Lys]-NH2, on human melanocortin receptors.使用模板促黑素肽Ac-Nle-c[Asp-His-Phe-Arg-Trp-Ala-Lys]-NH2对人黑皮质素受体进行立体化学修饰的生物学和构象研究。
J Med Chem. 1997 May 23;40(11):1738-48. doi: 10.1021/jm960845e.
9
Role of the alpha-amino group of protein in ubiquitin-mediated protein breakdown.蛋白质的α-氨基在泛素介导的蛋白质降解中的作用。
Proc Natl Acad Sci U S A. 1984 Nov;81(22):7021-5. doi: 10.1073/pnas.81.22.7021.
10
Three-dimensional molecular models of the hMC1R melanocortin receptor: complexes with melanotropin peptide agonists.人黑素皮质素受体1（hMC1R）的三维分子模型：与促黑素肽激动剂的复合物
Drug Des Discov. 1996 Dec;14(3):197-211.
引用本文的文献
1
Amino-terminal processing of proteins: hemoglobin South Florida, a variant with retention of initiator methionine and N alpha-acetylation.蛋白质的氨基末端加工：南佛罗里达血红蛋白，一种保留起始甲硫氨酸和Nα-乙酰化的变体。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8448-52. doi: 10.1073/pnas.82.24.8448.
2
Isolation of enzyme cDNA clones by enzyme immunodetection assay: isolation of a peptide acetyltransferase.通过酶免疫检测法分离酶cDNA克隆：肽酰基转移酶的分离
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1449-53. doi: 10.1073/pnas.84.5.1449.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
RAT LIVER ENZYME DOES NOT ACTIVATE ACETYLAMINO ACIDS.大鼠肝脏酶不会激活乙酰氨基酸。
J Biochem. 1965 Feb;57:176-83. doi: 10.1093/oxfordjournals.jbchem.a128074.
3
Chemical studies of histone acetylation. The occurrence of epsilon-N-acetyllysine in the f2a1 histone.组蛋白乙酰化的化学研究。f2a1组蛋白中ε-N-乙酰赖氨酸的存在。
J Biol Chem. 1968 Oct 10;243(19):5018-22.
4
Nonenzymatic acetylation of histones with acetyl-CoA.组蛋白与乙酰辅酶A的非酶促乙酰化作用。
Biochim Biophys Acta. 1970 Aug 8;213(2):513-22. doi: 10.1016/0005-2787(70)90058-4.
5
Extraction and partial purification of two histone-specific transacetylases from rat liver nuclei.从大鼠肝细胞核中提取并部分纯化两种组蛋白特异性转乙酰酶。
Biochem Biophys Res Commun. 1970 Jul 13;40(1):236-42. doi: 10.1016/0006-291x(70)91072-7.
6
N-acetyl-seryl-tRNA and polypeptide chain initiation during histone biosynthesis.组蛋白生物合成过程中的N-乙酰丝氨酰-tRNA与多肽链起始
Nature. 1970 May 2;226(5244):414-7. doi: 10.1038/226414a0.
7
N-terminal acetylation of the nascent chains of alpha-crystallin.
Biochem Biophys Res Commun. 1974 Jun 4;58(3):876-84. doi: 10.1016/s0006-291x(74)80498-5.
8
Synthesis of lens protein in vitro. N-terminal acetylation of alpha-crystallin.
Eur J Biochem. 1973 Sep 21;38(1):79-85. doi: 10.1111/j.1432-1033.1973.tb03036.x.
9
Synthesis of lens protein in vitro. Translation of calf-lens messengers in heterologous systems.晶状体蛋白的体外合成。小牛晶状体信使核糖核酸在异源系统中的翻译。
Eur J Biochem. 1973 Mar 15;33(3):551-7. doi: 10.1111/j.1432-1033.1973.tb02715.x.
10
Isolation of nuclear acidic proteins from rat tissues. Characterization of acetylated liver nuclear acidic proteins.从大鼠组织中分离核酸性蛋白。乙酰化肝细胞核酸性蛋白的特性分析。
Biochem J. 1974 Feb;137(2):355-62. doi: 10.1042/bj1370355.
Zotero 插件