Lebleu B, Sen G C, Shaila S, Cabrer B, Lengyel P
Proc Natl Acad Sci U S A. 1976 Sep;73(9):3107-11. doi: 10.1073/pnas.73.9.3107.
We reported earlier that the addition of double-stranded RNA and ATP increases the endonuclease activity more in an extract of Ehrlich ascites tumor cells which have been treated with an interferon preparation than in a comparable extract from control cells. We report here that the addition of double-stranded RNA to an extract from Ehrlich ascites tumor cells which have been treated with an interferon preparation [or with the interferon inducer poly(I)-poly(C)] promotes the phosphorylation by [gamma-32P]ATP of at least two proteins: P1 (molecular weight of 64,000) and P2 (molecular weight of 37,000). Double-stranded RNA also promotes the phosphorylation of at least one (i.e., P1) of these two proteins in an extract from cells which have not been treated with interferon, but the extent of phosphorylation is much smaller. Double-stranded RNA which has been degraded by RNase III, or DNA, does not promote the phosphorylation.
我们之前报道过，相较于来自对照细胞的类似提取物，在已用干扰素制剂处理过的艾氏腹水瘤细胞提取物中，添加双链RNA和ATP能更大程度地提高核酸内切酶活性。我们在此报道，向已用干扰素制剂[或干扰素诱导剂聚肌苷酸-聚胞苷酸（poly(I)-poly(C)）]处理过的艾氏腹水瘤细胞提取物中添加双链RNA，能促进[γ-32P]ATP对至少两种蛋白质的磷酸化作用：P1（分子量为64,000）和P2（分子量为37,000）。双链RNA还能促进未用干扰素处理过的细胞提取物中这两种蛋白质中至少一种（即P1）的磷酸化作用，但磷酸化程度要小得多。经核糖核酸酶III降解的双链RNA或DNA不能促进磷酸化作用。
