来自印度斯坦链霉菌的ShBle抗性决定簇在嗜盐栖热菌中表达并赋予对博来霉素的抗性。
The ShBle resistance determinant from Streptoalloteichus hindustanus is expressed in Haloferax volcanii and confers resistance to bleomycin.
作者信息
Nuttall S D, Deutschel S E, Irving R A, Serrano-Gomicia J A, Dyall-Smith M L
机构信息
CSIRO Health Sciences and Nutrition, and CRC for Diagnostic Technologies, 343 Royal Parade, Parkville, Victoria 3052, Australia.
出版信息
Biochem J. 2000 Mar 1;346 Pt 2(Pt 2):251-4.
Abstract
We have designed a gene cassette for expression of the bleomycin-resistance protein from Streptoalloteichus hindustanus (ShBle) in the extremely halophilic archaeon Haloferax volcanii, and shown that transformed haloarchaea are resistant to bleomycin. Recombinant ShBle was purified by a one-step affinity-chromatography procedure as a correctly folded, dimeric protein. ShBle thus provides a useful haloarchaeal selectable marker and represents the first non-halophilic and soluble heterologous protein to be expressed in the Haloarchaea.
摘要
我们设计了一个基因盒，用于在极端嗜盐古菌沃氏嗜盐栖热菌中表达来自印度游动放线菌的博来霉素抗性蛋白（ShBle），并证明转化后的嗜盐古菌对博来霉素具有抗性。重组ShBle通过一步亲和层析法纯化，得到正确折叠的二聚体蛋白。因此，ShBle提供了一个有用的嗜盐古菌选择标记，并且是第一个在嗜盐古菌中表达的非嗜盐可溶性异源蛋白。
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