Zhang H, Wu Z, Zhang Y
Department of General Surgery, Zhongshan Hospital, Shanghai Medical University.
Zhonghua Wai Ke Za Zhi. 1997 Sep;35(9):558-60.
We evaluated the preventive effects of glutamine-enriched pareneral nutrition on the atrophy and hypofunction of the transplanted small intestines in the rat. Wistar rats received jejunal isografts and central venous catheters for parenteral nutrition (PN) for ten days. Rats received either PN with 3% alanyl-glutamine or the PN with isonitrogenous ballanced nonessential amino acids. Glutamine-enriched PN significantly increased mucosal villous height mucosal thickness, crypt depth and villous surface area compared with the non-glutamine PN. Normal enterocyte ultrastructure of the graft was maintained in the Glutamine-enriched group. Atrophied microvilli and broken mitochondrial crista were observed in the control group. It was found that the amount of absorption of 15N-glycine was greater at the 1st, 2nd, 3rd hour in glutamine-enriched group than that in the control. These results indicate that the glutamine-enriched parenteral nutrition can promote the proliferation of mucosa, maintain the ultrastructure and improve the amino acid absorption in the transplanted small bowel in the rat.
我们评估了富含谷氨酰胺的肠外营养对大鼠移植小肠萎缩和功能减退的预防作用。Wistar大鼠接受空肠同种异体移植和中心静脉导管用于肠外营养（PN），为期10天。大鼠接受含3%丙氨酰谷氨酰胺的PN或含等氮平衡非必需氨基酸的PN。与不含谷氨酰胺的PN相比，富含谷氨酰胺的PN显著增加了黏膜绒毛高度、黏膜厚度、隐窝深度和绒毛表面积。富含谷氨酰胺组移植肠的肠上皮细胞超微结构保持正常。对照组观察到微绒毛萎缩和线粒体嵴断裂。发现富含谷氨酰胺组在第1、2、3小时15N-甘氨酸的吸收量高于对照组。这些结果表明，富含谷氨酰胺的肠外营养可促进大鼠移植小肠黏膜的增殖，维持超微结构并改善氨基酸吸收。
