Chiang W, Greaser M L, Lyons G E
Muscle Biology Laboratory, University of Wisconsin-Madison, Madison, WI 53706, USA.
Dev Dyn. 2000 Jan;217(1):99-108. doi: 10.1002/(SICI)1097-0177(200001)217:1<99::AID-DVDY9>3.0.CO;2-5.
The developmental pattern of filamin gene expression has been studied in mouse embryos by using in situ hybridization. The probes used were isoform specific, (35)S-labeled antisense complementary ribonucleic acids (cRNAs) to the 3; untranslated region (3; UTR) of muscle-specific and nonmuscle-specific filamin genes. Northern blot and in situ hybridization results showed that nonmuscle-specific filamin transcripts had a size of 9.5 kb and were expressed in all nonmuscle tissues. Labeling was most intense in tissues containing a substantial proportion of epithelial and smooth muscle cells. Muscle-specific filamin transcripts had a size of 10 kb and were expressed primarily in cardiac and skeletal muscle. The expression of muscle-specific filamin messenger ribonucleicacids (mRNAs) was detected in heart at 8.0 days after coitum, whereas that in the myotomes of somites was not detected until 10.5 days after coitum. The expression of muscle-specific filamin mRNAs in heart and in skeletal muscle continued through the subsequent days of myogenesis. The results showed that muscle-specific filamin gene transcripts are detected before the formation of myotubes in vivo. This is the first study of filamin gene expression at the early stages of skeletal muscle development. Dev Dyn 2000;217:99-108.
通过原位杂交技术，对小鼠胚胎中细丝蛋白基因表达的发育模式进行了研究。所用探针为亚型特异性的、用(35)S标记的针对肌肉特异性和非肌肉特异性细丝蛋白基因3′非翻译区(3′UTR)的反义互补核糖核酸(cRNAs)。Northern印迹和原位杂交结果显示，非肌肉特异性细丝蛋白转录本大小为9.5 kb，在所有非肌肉组织中均有表达。在含有大量上皮和平滑肌细胞的组织中标记最为强烈。肌肉特异性细丝蛋白转录本大小为10 kb，主要在心肌和骨骼肌中表达。在交配后8.0天，在心脏中检测到肌肉特异性细丝蛋白信使核糖核酸(mRNAs)的表达，而在体节的肌节中直到交配后10.5天才检测到。在随后的肌生成过程中，心脏和骨骼肌中肌肉特异性细丝蛋白mRNAs的表达持续存在。结果表明，在体内肌管形成之前就能检测到肌肉特异性细丝蛋白基因转录本。这是骨骼肌发育早期细丝蛋白基因表达的首次研究。《发育动力学》2000年；217:99 - 108。
