Madhusudanan K P, Katti S B, Haq W, Misra P K
Central Drug Research Institute, Lucknow 226001, India.
J Mass Spectrom. 2000 Feb;35(2):237-41. doi: 10.1002/(SICI)1096-9888(200002)35:2<237::AID-JMS934>3.0.CO;2-3.
Non-covalent interactions between met- and leu-enkephalins and their antisense peptides were studied by electrospray ionization mass spectrometry. Mixtures of sense and antisense peptides gave both the corresponding homodimers and heterodimers. The relative abundance ratios of the heterodimer to that of the homodimer of the sense peptide and the relative stability constants of the heterodimers were compared with the corresponding values from mixtures of the sense peptides and a control peptide. The results show that there is a preferential interaction between the sense and antisense peptides compared with that between the sense and control peptides.
通过电喷雾电离质谱法研究了甲硫氨酸脑啡肽和亮氨酸脑啡肽与其反义肽之间的非共价相互作用。有义肽和反义肽的混合物产生了相应的同二聚体和异二聚体。将有义肽异二聚体与同二聚体的相对丰度比以及异二聚体的相对稳定性常数与有义肽和对照肽混合物的相应值进行了比较。结果表明，与有义肽和对照肽之间的相互作用相比，有义肽和反义肽之间存在优先相互作用。
