Yang Y, Cao Z, Han Z
Si Chuan Provincial Hospital, ChengDu.
Zhonghua Fu Chan Ke Za Zhi. 1998 May;33(5):268-70.
To study the effects of mifepristone on proliferation and apoptosis in early pregnant chorionic villi.
Proliferation and apoptosis in early pregnant choionic villi from surgical aspiration and mifepristone induced abortion were studied. Marker for proliferation is proliferating cell nuclear antigen (PCNA) immunohistochemical staining, apoptotic cell death was detected using DNA in situ terminal deoxynucleotidyl transferas-mediated dUTP-biotin nick ending labeling (TUNEL).
In early pregnant chorionic villi, proliferating index of cytotrophoblasts was 53.74% +/- 1.34%, not only nuclear but also cytoplasma were PCNA positive staining, syncytiotrophoblasts were negative. There existed apoptotic cell death in trophoblasts, the apoptotic index were 2.52% +/- 0.86% in syncytiotrophoblasts and 0.52% +/- 0.26% in cytotrophblasts, respectively. After 2 days mifepristone treatment, the proliferating index of cytotrophoblasts decreased slightly and PCNA positive cytoplasmic expression disappeared; while apoptotic cell increased significantly, the apoptotic indices were 22.16% +/- 2.26% in syncytiotrophoblasts and 20.12% +/- 1.74% in cytotrophoblasts, respectively.
Mifepristone may inhibit proliferation and promote apoptosis of trophoblasts in early pregnant chorionic villi.
研究米非司酮对早孕绒毛增殖和凋亡的影响。
研究手术清宫及米非司酮药物流产的早孕绒毛的增殖和凋亡情况。增殖标志物采用增殖细胞核抗原（PCNA）免疫组化染色，凋亡细胞死亡采用脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（TUNEL）检测。
早孕绒毛中，细胞滋养层细胞的增殖指数为53.74%±1.34%，细胞核及细胞质均呈PCNA阳性染色，合体滋养层细胞呈阴性。滋养层细胞存在凋亡，合体滋养层细胞凋亡指数为2.52%±0.86%，细胞滋养层细胞凋亡指数为0.52%±0.26%。米非司酮处理2天后，细胞滋养层细胞增殖指数略有下降，PCNA阳性细胞质表达消失；而凋亡细胞显著增加，合体滋养层细胞凋亡指数分别为22.16%±2.26%，细胞滋养层细胞凋亡指数为20.12%±1.74%。
米非司酮可能抑制早孕绒毛滋养层细胞的增殖并促进其凋亡。
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