McCaughan H M, McRae R Z, Smith H K
Department of Sport and Exercise Science, The University of Auckland, New Zealand.
Int J Sports Med. 2000 Jan;21(1):37-40. doi: 10.1055/s-2000-8849.
This study aimed to determine the stability of lactate concentration in blood samples preserved and stored using methods practical for field testing and experimental applications. Whole blood microsamples were obtained from venous samples drawn from 10 healthy subjects following bouts of moderate (approximately 5 mmol x l(-1), n = 12), or intense (approximately 10 mmol x l(-1), n = 12), treadmill exercise. Samples were analysed fresh (2 x 25 microl), or placed in preservative-containing tubes (12 x 75 microl) and analysed directly, or after storage at room temperature (RT) or 4 degrees C, for 1 h, 18 h, 2 d, 3 d or 7 d, or at -20 degrees C for 7 d. In comparison to preserved samples assayed directly after collection, lactate levels in all RT samples had declined significantly, whereas the 4 degrees C samples had not changed, by 2 d post-collection. After 7 d of refrigeration, the absolute value of the difference from lactate levels in samples measured after collection (mean +/- SD) was 0.38 +/- 0.34 mmol x l(-1), or 5.3 +/- 4.3%; with freezing, this difference was 0.27 +/- 0.27 mmol x l(-1), or 3.6 +/- 3.0%. These differences were less than the daily variation in the analyser readings of a 10 mmol x l(-1) standard, indicating that the blood preservation and storage methods identified herein are suitable for use during exercise testing.
本研究旨在确定采用适用于现场检测和实验应用的方法保存和储存的血液样本中乳酸浓度的稳定性。从10名健康受试者进行中度（约5 mmol·L⁻¹，n = 12）或剧烈（约10 mmol·L⁻¹，n = 12）跑步机运动后抽取的静脉样本中获取全血微量样本。样本分为新鲜分析组（2×25 μl），或置于含防腐剂的试管中（12×75 μl），直接分析，或在室温（RT）或4℃下储存1小时、18小时、2天、3天或7天，或在-20℃下储存7天后分析。与采集后直接检测的保存样本相比，所有室温样本中的乳酸水平均显著下降，而4℃样本在采集后2天内未发生变化。冷藏7天后，与采集后测量的样本中乳酸水平的差值绝对值（均值±标准差）为0.38±0.34 mmol·L⁻¹，或5.3±4.3%；冷冻后，该差值为0.27±0.27 mmol·L⁻¹，或3.6±3.0%。这些差异小于10 mmol·L⁻¹标准品分析仪读数的每日变化，表明本文确定的血液保存和储存方法适用于运动测试。
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