Tas O, De Rooster H, Baert E, Doom M H, Duchateau L
Department of Medicine and Clinical Biology of Small Animals, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium.
J Small Anim Pract. 2008 Oct;49(10):504-8. doi: 10.1111/j.1748-5827.2008.00668.x. Epub 2008 Oct 1.
The objective of this study was to determine the accuracy of the Lactate Pro hand-held analyser in measuring blood lactate levels.
Blood was drawn from 15 healthy dogs into five tubes containing Na-EDTA. Lactate was measured immediately using the Lactate Pro analyser and a laboratory reference method. Further samples were analysed 120, 240, 480 and 1440 minutes later to artificially increase the lactate levels. Lactate was measured in blood samples of 60 healthy dogs using the Lactate Pro analyser to determine the reference interval of lactate concentration in normal dogs.
The correlation between the lactate concentration measured with the Lactate Pro analyser and the reference method was high. Lactate levels were lower when measured with the hand-held analyser than with the traditional laboratory determination. The reference interval for blood lactate concentrations in healthy dogs established by the Lactate Pro analyser was from the detection limit (0.8 mmol/l) up to 3.3 mmol/l.
The Lactate Pro analyser provides quick and reliable measurements of blood lactate in dogs with blood lactate levels up to 10 mmol/l. Because of its small sample size, this analyser will be particularly appropriate for use in small animal intensive care.
本研究的目的是确定乳酸便携分析仪测量血乳酸水平的准确性。
从15只健康犬采集血液,放入五支含乙二胺四乙酸钠的试管中。立即使用乳酸便携分析仪和实验室参考方法测量乳酸。120、240、480和1440分钟后对另外的样本进行分析,以人为提高乳酸水平。使用乳酸便携分析仪测量60只健康犬的血液样本中的乳酸,以确定正常犬乳酸浓度的参考区间。
乳酸便携分析仪测量的乳酸浓度与参考方法之间的相关性很高。用手持分析仪测量的乳酸水平低于传统实验室测定法。乳酸便携分析仪确定的健康犬血乳酸浓度参考区间为从检测限(0.8毫摩尔/升)至3.3毫摩尔/升。
乳酸便携分析仪能快速、可靠地测量血乳酸水平高达10毫摩尔/升的犬的血乳酸。由于其样本量小,该分析仪特别适用于小动物重症监护。