Towler E M, Wilson L K, Zhou Y C, Ma T S, Fisher R J
SAIC Frederick, NCI-FCRDC, Frederick, Maryland, 21702, USA.
Anal Biochem. 2000 Mar 1;279(1):96-9. doi: 10.1006/abio.1999.4449.
We have developed a complete system for discovery of lead compounds as inhibitors of creatine kinase B. In this article, we describe production and purification of the recombinant protein, conditions and features of an optimized high-throughput screening assay, and results of our implementation of the system using a diverse compound library.
我们已经开发出了一套完整的系统，用于发现作为肌酸激酶B抑制剂的先导化合物。在本文中，我们描述了重组蛋白的生产与纯化、优化的高通量筛选试验的条件和特点，以及我们使用多样化化合物库实施该系统的结果。
