Chen W, Cheng D, Wu Q
Department of Internal Medicine, First Affiliated Hospital, Chengdu.
Hua Xi Yi Ke Da Xue Xue Bao. 1998 Sep;29(3):233-6.
To observe the effect of chronic hypoxia on the gene expression of vascular endothelial growth factor (VEGF) in rat's lung, and the role of VEGF in the pathogenesis of hypoxic pulmonary hypertension, thirteen male Wistar rats were exposed to isobaric hypoxia for 3 weeks. The pulmonary artery pressure was measured by right cardiac catheterization. The serum level of VEGF was measured by Elisa. The VEGF cRNA was labeled with digoxigenin-UTP by in vitro transcription. The expression of VEGF mRNA in the lung was examined by hybridization in situ. The pulmonary artery pressure was significantly increased after hypoxic exposure. The serum level of VEGF in rats treated with hypoxia (420.3 +/- 73.1 pg/ml) was significantly increased in comparison with that of normal rats (322.2 +/- 58.1 pg/ml). The VEGF hybridization signals on the wall of pulmonary arteriole were significantly increased in rats with pulmonary hypertension. Chronic hypoxia can markedly increase expression of VEGF mRNA in the pulmonary arteriole and hence stimulate VEGF synthesis and secretion. The increase of VEGF may play a role in the developing process of hypoxic pulmonary hypertension.
为观察慢性缺氧对大鼠肺组织血管内皮生长因子（VEGF）基因表达的影响，以及VEGF在缺氧性肺动脉高压发病机制中的作用，将13只雄性Wistar大鼠置于等压缺氧环境中3周。采用右心导管法测量肺动脉压。用酶联免疫吸附测定法（ELISA）检测血清VEGF水平。通过体外转录用洋地黄毒苷-UTP标记VEGF cRNA。采用原位杂交法检测肺组织中VEGF mRNA的表达。缺氧暴露后肺动脉压显著升高。与正常大鼠（322.2±58.1 pg/ml）相比，缺氧处理大鼠的血清VEGF水平（420.3±73.1 pg/ml）显著升高。肺动脉高压大鼠肺小动脉壁上的VEGF杂交信号显著增加。慢性缺氧可显著增加肺小动脉中VEGF mRNA的表达，从而刺激VEGF的合成与分泌。VEGF的增加可能在缺氧性肺动脉高压的发生发展过程中起作用。
