α7整合素缺陷小鼠轴突再生受损。
Impaired axonal regeneration in alpha7 integrin-deficient mice.
作者信息
Werner A, Willem M, Jones L L, Kreutzberg G W, Mayer U, Raivich G
机构信息
Department of Neuromorphology, Max-Planck-Institute of Neurobiology, 82152 Martinsried, Germany.
出版信息
J Neurosci. 2000 Mar 1;20(5):1822-30. doi: 10.1523/JNEUROSCI.20-05-01822.2000.
Abstract
The interplay between growing axons and the extracellular substrate is pivotal for directing axonal outgrowth during development and regeneration. Here we show an important role for the neuronal cell adhesion molecule alpha7beta1 integrin during peripheral nerve regeneration. Axotomy led to a strong increase of this integrin on regenerating motor and sensory neurons, but not on the normally nonregenerating CNS neurons. alpha7 and beta1 subunits were present on the axons and their growth cones in the regenerating facial nerve. Transgenic deletion of the alpha7 subunit caused a significant reduction of axonal elongation. The associated delay in the reinnervation of the whiskerpad, a peripheral target of the facial motor neurons, points to an important role for this integrin in the successful execution of axonal regeneration.
摘要
在发育和再生过程中，生长中的轴突与细胞外基质之间的相互作用对于引导轴突生长至关重要。在此，我们展示了神经元细胞粘附分子α7β1整合素在周围神经再生过程中的重要作用。轴突切断术导致这种整合素在再生的运动和感觉神经元上显著增加，但在正常情况下不发生再生的中枢神经系统神经元上则没有增加。α7和β1亚基存在于再生面神经的轴突及其生长锥上。α7亚基的转基因缺失导致轴突伸长显著减少。面部运动神经元的外周靶标——触须垫再支配的相关延迟表明，这种整合素在轴突再生的成功执行中发挥着重要作用。
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