Sandler S G, Langeberg A, Avery N, Mintz P D
Department of Laboratory Medicine, Georgetown University Medical Center, Washington, DC 20007, USA.
Transfusion. 2000 Feb;40(2):201-7. doi: 10.1046/j.1537-2995.2000.40020201.x.
The results of routine blood bank testing by a fully automated blood typing system (ABS2000) were compared with those obtained by standard manual methods in six hospital transfusion services.
The ABS2000 system uses microtiter plates for determining ABO and D types, solid-phase red cell adherence (SPRCA) assays for antibody detection, and modified SPRCA plates for IgG crossmatches. The transfusion services used their standard manual test tube methods.
Of 3779 donors' samples tested for ABO types (red cell typings only), 3.0 percent could not be interpreted by the ABS2000 system's neural network, because of clots, hemolysis, or lipemic samples. The results for ABO types were concordant for 99.8 percent of the remaining samples. Of 3779 donors' samples tested for D types, the results were concordant for 98.7 percent. Of 7580 patients' samples tested for ABO types (red cell and plasma typings), 5.8 percent could not be interpreted by the ABS2000 system. There was 100-percent concordance of ABO typing results for the remaining 7140 samples. There was 99. 7-percent concordance of results for patients' D types. The results of 96.7 percent of antibody detection tests and 98.8 percent of crossmatches were concordant. Neither method failed to detect a serologically incompatible crossmatch that was associated with a specific, clinically significant alloantibody. The ABS2000 system performed 45 confirmatory donor ABO and D types in 115 minutes, 22 antibody detection tests in 116 minutes, 16 patients' ABO/D types in 149 minutes, and 40 crossmatches in 140 minutes.
The ABS2000 blood typing system automates routine blood bank tests with accuracy comparable to that of hospital transfusion services' standard manual methods.
在六家医院输血服务机构中，将全自动血型系统（ABS2000）的常规血库检测结果与标准手工方法获得的结果进行了比较。
ABS2000系统使用微量滴定板确定ABO和D血型，采用固相红细胞黏附（SPRCA）试验检测抗体，并使用改良的SPRCA板进行IgG交叉配血。输血服务机构使用其标准的手工试管法。
在检测ABO血型（仅红细胞分型）的3779份供者样本中，由于存在凝块、溶血或脂血样本，3.0%的样本无法通过ABS2000系统的神经网络进行解读。其余样本中，ABO血型结果的一致性为99.8%。在检测D血型的3779份供者样本中,结果的一致性为98.7%。在检测ABO血型（红细胞和血浆分型）的7580份患者样本中，5.8%的样本无法通过ABS2000系统进行解读。其余7140份样本的ABO血型分型结果一致性为100%。患者D血型结果的一致性为99.7%。抗体检测试验结果的一致性为96.7%，交叉配血结果的一致性为98.8%。两种方法均未漏检与特定的、具有临床意义的同种抗体相关的血清学不相容交叉配血。ABS2000系统在115分钟内完成了45次供者ABO和D血型的确证试验，在116分钟内完成了22次抗体检测试验，在149分钟内完成了16例患者的ABO/D血型检测，在140分钟内完成了40次交叉配血。
ABS2000血型系统使常规血库检测自动化，其准确性与医院输血服务机构的标准手工方法相当。
