从纤维素分解梭菌菌株MCF1中检测、克隆和分析一种本地质粒。

Detection, cloning, and sequence analysis of an indigenous plasmid from cellulolytic clostridial strain MCF1.

作者信息

Chen T, Ouko L, Warnick T, Leschine S

机构信息

Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, 01003-5720, USA.

出版信息

Plasmid. 2000 Mar;43(2):153-8. doi: 10.1006/plas.1999.1439.

DOI:10.1006/plas.1999.1439

PMID:10686135

Abstract

Nucleotide sequence analysis of a 2451-bp plasmid (pMCF1) from a cellulolytic Clostridium revealed that the protein specified by the largest open reading frame (ORF1) was homologous to RepB of Clostridium butyricum plasmid pCB101. The data suggest that pMCF1 belongs to the pC194 family of rolling-circle replicating plasmids and the ORF1 protein functions as its replication protein.

摘要

对来自一株纤维素分解梭菌的2451 bp质粒（pMCF1）进行核苷酸序列分析发现，最大开放阅读框（ORF1）所编码的蛋白质与丁酸梭菌质粒pCB101的RepB同源。数据表明，pMCF1属于滚环复制质粒的pC194家族，且ORF1蛋白作为其复制蛋白发挥作用。