Miida T, Ozaki K, Murakami T, Kashiwa T, Yamadera T, Tsuda T, Inano K, Okada M
Department of Laboratory Medicine, Niigata University School of Medicine, Asahimachi 1-757, Niigata, Japan.
Clin Chim Acta. 2000 Feb 25;292(1-2):69-80. doi: 10.1016/s0009-8981(99)00259-4.
To clarify whether prebeta1-high-density lipoprotein (prebeta1-HDL) concentration changes with low-density lipoprotein-cholesterol (LDL-C) concentration independent of cholesteryl ester transfer protein (CETP), we determined prebeta1-HDL concentration by native two-dimensional gel electrophoresis in 58 subjects with normal triglyceride and HDL-cholesterol concentrations. We also measured LDL-C and CETP concentrations. In 17 subjects, a second blood sample was taken 1-6 months after the first. We found that prebeta1-HDL concentration was positively correlated with LDL-C concentration (r=0.529, P<0.0001) and with CETP mass (r=0.398, P<0.01). In 17 patients, Deltaprebeta1-HDL was positively correlated with DeltaLDL-C (r=0.635, P<0.01), but not with DeltaCETP mass (r=0.275). In conclusion, prebeta1-HDL concentration changes with LDL-C concentration independent of CETP. These results suggest that prebeta1-HDL concentration may reflect the balance between several regulatory factors, including LDL-C and CETP concentrations.
为了阐明前β1-高密度脂蛋白（prebeta1-HDL）浓度是否随低密度脂蛋白胆固醇（LDL-C）浓度变化，且独立于胆固醇酯转运蛋白（CETP），我们通过天然二维凝胶电泳法测定了58名甘油三酯和HDL-胆固醇浓度正常的受试者的prebeta1-HDL浓度。我们还测量了LDL-C和CETP浓度。在17名受试者中，在首次采血后1 - 6个月采集了第二份血样。我们发现prebeta1-HDL浓度与LDL-C浓度呈正相关（r = 0.529，P < 0.0001），与CETP质量呈正相关（r = 0.398，P < 0.01）。在17名患者中，Delta prebeta1-HDL与Delta LDL-C呈正相关（r = 0.635，P < 0.01），但与Delta CETP质量无关（r = 0.275）。总之，prebeta1-HDL浓度随LDL-C浓度变化，且独立于CETP。这些结果表明，prebeta1-HDL浓度可能反映了包括LDL-C和CETP浓度在内的几种调节因子之间的平衡。
