Cardoso C L, Kotaka C R, Redmerski R, Guilhermetti M, Queiroz A F
Department of Clinical Analyses, State University of Maringá, Paraná, Brazil.
J Endod. 1999 Jul;25(7):498-501. doi: 10.1016/S0099-2399(99)80290-8.
Gutta-percha cones are now widely used to fill root canals. Because they cannot be sterilized by conventional autoclaving or in a hot-air oven, gutta-percha cones require rapid chairside decontamination before use to maintain the aseptic chain, an essential factor in successful endodontic therapy. The purpose of this study was to compare the effectiveness of different concentrations of sodium hypochlorite (0.25% to 4%) in sterilizing gutta-percha cones artificially contaminated with Staphylococcus aureus and Escherichia coli strains, and Bacillus subtilis spores. After 1 min of treatment, the solutions tested showed bactericidal and sporicidal effects at concentrations of 0.25% and 1%, respectively. At a concentration of 0.25%, the solutions tested were effective in destroying spores after 5 min of exposure. Based on this study, treatment of the cones for 1 min with 1% sodium hypochlorite (Milton's solution) or for 5 min with Dakin's liquid (0.5% sodium hypochlorite) is recommended.
牙胶尖目前被广泛用于充填根管。由于牙胶尖不能通过传统的高压灭菌或在热风烘箱中进行消毒，因此在使用前需要在椅旁快速进行去污处理，以维持无菌流程，这是成功进行牙髓治疗的一个关键因素。本研究的目的是比较不同浓度的次氯酸钠（0.25%至4%）对人工污染有金黄色葡萄球菌、大肠杆菌菌株和枯草芽孢杆菌孢子的牙胶尖的消毒效果。经过1分钟的处理后，所测试的溶液在浓度分别为0.25%和1%时显示出杀菌和杀孢子作用。在浓度为0.25%时，所测试的溶液在暴露5分钟后能够有效破坏孢子。基于本研究，建议用1%的次氯酸钠（米尔顿溶液）对牙胶尖处理1分钟，或用达金氏液（0.5%次氯酸钠）处理5分钟。
