Grimaldi C, Dutertre M, Simonet J M
Institut de Génétique et Microbiologie, Centre National de Recherche Scientifique, Unité Mixte de Recherche 8621, Université Paris-Sud, Bâtiment 360, 91 405 Orsay Cedex, France.
Curr Microbiol. 2000 Apr;40(4):245-9. doi: 10.1007/s002849910049.
The guaA gene encoding GMP synthetase was cloned from a potential probiotic strain of Lactobacillus rhamnosus. DNA sequence and Northern blot analysis indicated that (i) guaA did not belong to an guaAB operonic structure, conversely to enteric bacteria, (ii) L. rhamnosus guaA seemed to be highly expressed, and (iii) genetic regulation might differ from Bacillus subtilis. Moreover, differences in the genetic organization of guaA allowed discrimination of some closely related L. rhamnosus strains, with a rapid screening by PCR.
从鼠李糖乳杆菌的一株潜在益生菌中克隆了编码GMP合成酶的guaA基因。DNA序列和Northern印迹分析表明：（i）与肠道细菌相反，guaA不属于guaAB操纵子结构；（ii）鼠李糖乳杆菌guaA似乎高度表达；（iii）其基因调控可能与枯草芽孢杆菌不同。此外，guaA基因组织的差异使得可以区分一些密切相关的鼠李糖乳杆菌菌株，并通过PCR进行快速筛选。
