Champavier Y, Pommier M, Arpin N, Voiland A, Pellon G
Laboratoire de Mycochimie, Unité de Formation et de Recherche de Chimie-Biochimie, Université Claude Bernard, 43 bd du 11 Novembre 1918, 69622, Villeurbanne Cedex, France
Enzyme Microb Technol. 2000 Feb 1;26(2-4):243-251. doi: 10.1016/s0141-0229(99)00139-8.
The few well-characterized fungal growth-regulating substances include 10-oxo-trans-8-decenoic acid (ODA) and hercynine. This report deals with production and tissue location of ODA. It also describes some biological activities of addition of ODA, hercynine, and cytokinins on growth and postharvest morphogenesis of Agaricus bisporus. Production of ODA in sporophore extracts was limited mainly by oxygen availability and the possible occurrence of a competitive metabolic pathway. Presumably synthesized within the stipe and skin tissues, ODA accumulated in the gills. Mycelium growth rate on a potato-based medium was significantly increased in the presence of ODA. Moreover, stipe lengthening was slightly stimulated by 10 or 100 µM ODA. Although these findings were similar to previous ones (Mau JL, Beelman RB, Ziegler GR. Phytochemistry 1992;31:4059-64), ODA appeared poorly active in our assays and mycelium growth on asparagine-glucose medium was strongly inhibited by 200 µM ODA. In contrast with cytokinins or hercynine, ODA did not speed up cap opening. Finally, tests carried out on animal cells suggested a dose-dependent cytotoxic effect of ODA.
少数已明确特性的真菌生长调节物质包括10-氧代-反式-8-癸烯酸（ODA）和组氨酸。本报告涉及ODA的产生及组织定位。它还描述了添加ODA、组氨酸和细胞分裂素对双孢蘑菇生长和采后形态发生的一些生物学活性。子实体提取物中ODA的产生主要受氧供应和可能存在的竞争性代谢途径限制。ODA大概在菌柄和表皮组织中合成，在菌褶中积累。在含ODA的情况下，马铃薯培养基上的菌丝体生长速率显著提高。此外，10或100μM的ODA对菌柄伸长有轻微刺激作用。尽管这些发现与之前的研究结果相似（Mau JL，Beelman RB，Ziegler GR。植物化学，1992年；31：4059 - 64），但在我们的试验中ODA活性似乎较差，200μM的ODA强烈抑制了天冬酰胺 - 葡萄糖培养基上的菌丝体生长。与细胞分裂素或组氨酸不同，ODA并未加速菌盖张开。最后，对动物细胞进行的测试表明ODA具有剂量依赖性细胞毒性作用。
