Nam B H, Yamamoto E, Hirono I, Aoki T
Laboratory of Genetics and Biochemistry, Department of Aquatic Biosciences, Tokyo University of Fisheries, Konan 4-5-7, Minato, Tokyo, Japan.
Dev Comp Immunol. 2000 Jan;24(1):13-24. doi: 10.1016/s0145-305x(99)00058-0.
We constructed a cDNA library of Japanese flounder, Paralichthys olivaceus, leukocytes that were infected with Hirame rhabdovirus (HRV) in order to analyze some of the genes that are induced and expressed by virus infection in the immune system. Four hundred and fifty-two partial sequences representing 300 cDNA clones were obtained from the 5' and/or 3' ends of inserts derived from the Japanese flounder leukocyte cDNA library. About three-quarters of the 300 cDNA clones (217 clones, 72.3%) represented known genes in the public databases, whereas the remaining 83 (27.7%) of the clones did not show any significant homology with the sequences in the public databases. Clones matching known genes were classified into 12 categories according to their function or distribution. Only 40 (18.4%) of the 217 known genes showed homology with fish genes deposited in the database. Thirty (10%) of the clones, encoding 21 different sequences, and representing several categories, were identified as putative biodefense genes or genes associated with the immune response. Nineteen of the 21 putative biodefense or immune response-related cDNAs have not been previously reported in fish genes or cDNAs.
为了分析免疫系统中由病毒感染诱导和表达的一些基因，我们构建了受牙鲆弹状病毒（HRV）感染的牙鲆（Paralichthys olivaceus）白细胞的cDNA文库。从牙鲆白细胞cDNA文库的插入片段的5'和/或3'末端获得了代表300个cDNA克隆的452个部分序列。300个cDNA克隆中约四分之三（217个克隆，72.3%）代表公共数据库中的已知基因，而其余83个（27.7%）克隆与公共数据库中的序列没有任何显著同源性。与已知基因匹配的克隆根据其功能或分布分为12类。217个已知基因中只有40个（18.4%）与数据库中存储的鱼类基因具有同源性。30个克隆（10%）编码21种不同序列，代表几个类别，被鉴定为推定的生物防御基因或与免疫反应相关的基因。21个推定的生物防御或免疫反应相关cDNA中有19个以前在鱼类基因或cDNA中未被报道过。
