Mallet B, Gulian J M, Sciaky M, Laurent G, Charrel M
Biochim Biophys Acta. 1979 Feb 26;576(2):290-304. doi: 10.1016/0005-2795(79)90404-5.
Three major forms CI, CII and CX and the minor component CI1v of ovine erythrocyte carbonic anhydrase have been identified in the ethanol chloroform extract of the hemolysate by electrophoresis on cellulose acetate and isoelectrofocusing. Isolation of the four forms was achieved by chromatography on DEAE-Sephadex A-50. Comparative studies suggest that: (1) form CI1v is a modified form of CI, (2) form CX is CI plus glutathione and (3) forms CI and CII are isoenzymes which differ in their primary structure by one Lys leads to Thr substitution 35 residues from the N-acetylated terminus.
通过醋酸纤维素电泳和等电聚焦法,在溶血产物的乙醇氯仿提取物中鉴定出绵羊红细胞碳酸酐酶的三种主要形式CI、CII和CX以及次要成分CI1v。通过DEAE-葡聚糖A-50柱色谱法实现了这四种形式的分离。比较研究表明:(1)CI1v形式是CI的一种修饰形式,(2)CX形式是CI加谷胱甘肽,(3)CI和CII形式是同工酶,它们的一级结构在距N-乙酰化末端35个残基处因一个赖氨酸被苏氨酸取代而有所不同。