Tsiligianni T, Karagiannidis A, Brikas P, Saratsis P
Clinic of Obstetrics and AI, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Greece.
Dtsch Tierarztl Wochenschr. 2000 Jan;107(1):28-31.
The object of the present investigation was to determine several physical properties of cervical mucus in spontaneous oestrus cows in relation to fertility. Because, it is very difficult to determine the exact time of the beginning of oestrus in cows, the aim of the present study was to investigate if physical properties of cervical mucus at the time of artificial insemination (AI) are related to conception or not. A total of 93 cows of Friesian breed were used. The animals exhibited spontaneous oestrus, without being submitted to any hormonal treatment. Samples of cervical mucus were collected 5-30 min before AI and pH, viscosity, spinnbarkeit (spinability), crystallization and penetration were measured. Pregnancy diagnosis was performed by rectal palpation 3 months later. The results obtained from cows that conceived (44 animals), compared to those obtained from cows that did not conceive (49 animals), were the following: a) viscosity had been significantly lower (p < 0.05), b) crystallization had been significantly higher (p < 0.05), and c) pH, spinnbarkeit and penetration of spermatozoa into cervical mucus did not differ. In conclusion, the best time for AI is when viscosity is below 20 mm H2O and crystallization is above 3. Viscosity and crystallization could be related to ovulation time, but this needs further investigation.
本研究的目的是确定自然发情奶牛宫颈黏液的几种物理特性与生育力之间的关系。因为很难确定奶牛发情开始的确切时间，所以本研究的目的是调查人工授精（AI）时宫颈黏液的物理特性是否与受孕有关。总共使用了93头弗里生品种的奶牛。这些动物表现出自然发情，未接受任何激素处理。在人工授精前5 - 30分钟采集宫颈黏液样本，并测量其pH值、黏度、拉丝度、结晶情况和精子穿透能力。3个月后通过直肠触诊进行妊娠诊断。受孕奶牛（44头）与未受孕奶牛（49头）的结果如下：a）黏度显著较低（p < 0.05），b）结晶显著较高（p < 0.05），c）pH值、拉丝度和精子穿透宫颈黏液的能力没有差异。总之，人工授精的最佳时间是黏度低于20毫米水柱且结晶高于3时。黏度和结晶可能与排卵时间有关，但这需要进一步研究。
