Komano T
Department of Biology, Tokyo Metropolitan University, Japan.
Annu Rev Genet. 1999;33:171-91. doi: 10.1146/annurev.genet.33.1.171.
Conservative site-specific recombination functions to create biological diversity in prokaryotes. Simple site-specific recombination systems consist of two recombination sites and a recombinase gene. The plasmid R64 shufflon contains seven recombination sites, which flank and separate four DNA segments. Site-specific recombinations mediated by the product of the rci gene between any two inverted recombination sites result in the inversion of four DNA segments independently or in groups. The shufflon functions as a biological switch to select one of seven C-terminal segments of the PilV proteins, which is a minor component of R64 thin pilus. The shufflon determines the recipient specificity in liquid matings of plasmid R64. Other multiple inversion systems as well as integrons, which are multiple insertion systems, are also described in this review.
保守性位点特异性重组作用是在原核生物中创造生物多样性。简单的位点特异性重组系统由两个重组位点和一个重组酶基因组成。质粒R64可变区含有七个重组位点，这些位点位于四个DNA片段两侧并将其隔开。由rci基因产物介导的任何两个反向重组位点之间的位点特异性重组会导致四个DNA片段独立或成组地发生倒位。可变区作为一种生物开关，用于选择PilV蛋白七个C端片段中的一个，PilV蛋白是R64细菌毛的次要成分。可变区决定了质粒R64液体交配中的受体特异性。本综述还描述了其他多重倒位系统以及整合子，整合子是多重插入系统。
