Gunin A G, Sharov A A, Nikolaev D V
Department of Histology, Medical Institute Chuvash State University, Cheboksary, Russia.
Eur J Obstet Gynecol Reprod Biol. 2000 Feb;88(2):171-9. doi: 10.1016/s0301-2115(99)00136-0.
The present work was undertaken to examine the effect of 2 month glucocorticoid treatment on estradiol-induced proliferation in the uterus. Ovariectomized rats were treated with long-acting triamcinolone acetonide or saline for 60 days followed by a single injection of estradiol dipropionate or vehicle. Proliferation in the uterus was estimated by the mitotic index, bromodeoxyuridine-labelling index and proliferating cells nuclear antigen-labelling index 24, 36 and 48 h after the injection of estradiol or vehicle. Two month glucocorticoid treatment resulted in an initial decrease in all the parameters for luminal and glandular epithelia followed by an increase to 48 h after estradiol injection, and in a large increase in all proliferative parameters for stromal and myometrial cells at all periods of observation, compared with control rats untreated with glucocorticoid. The 2 month glucocorticoid treatment protocol had no significant effect on the parameters without estradiol administration. Analysis of proliferation for myometrial cells was also performed after 1 month treatment with glucocorticoid followed by estradiol or saline. Results showed no significant influence of 1 month treatment with glucocorticoid on myometrial cells.
本研究旨在探讨为期2个月的糖皮质激素治疗对雌二醇诱导的子宫增殖的影响。对去卵巢大鼠用长效曲安奈德或生理盐水治疗60天，随后单次注射二丙酸雌二醇或赋形剂。在注射雌二醇或赋形剂后24、36和48小时，通过有丝分裂指数、溴脱氧尿苷标记指数和增殖细胞核抗原标记指数评估子宫中的增殖情况。与未用糖皮质激素治疗的对照大鼠相比，为期2个月的糖皮质激素治疗导致腔上皮和腺上皮的所有参数最初下降，随后在注射雌二醇后48小时增加，并且在所有观察期基质和肌层细胞的所有增殖参数大幅增加。为期2个月的糖皮质激素治疗方案对未给予雌二醇时的参数无显著影响。在用糖皮质激素治疗1个月后再给予雌二醇或生理盐水，也对肌层细胞进行了增殖分析。结果显示，为期1个月的糖皮质激素治疗对肌层细胞无显著影响。
