Katz J, Dewald R, Nicholson J
Diagnostic Bacteriology Laboratory, National Veterinary Services Laboratories, US Department of Agriculture, Animal and Health Inspection Services, Ames, IA 50010, USA.
J Vet Diagn Invest. 2000 Jan;12(1):46-50. doi: 10.1177/104063870001200108.
Procedurally similar competitive enzyme-linked immunoassay (cELISA) methods were developed for the serodiagnosis of Babesia equi and Babesia caballi (piroplasmosis), Trypanosoma equiperdum (dourine), and Burkholderia mallei (glanders) infections in horses. Apparent test specificities for the B. equi, B. caballi, T. equiperdum, and B. mallei cELISAs were 99.2%, 99.5%, 98.9%, and 98.9%, respectively. Concordances and kappa values between the complement fixation (CF) and the cELISA procedures for the serodiagnosis of B. equi, B. caballi, T. equiperdum, and B. mallei infections in experimentally exposed horses were 76% and 0.55, 89% and 0.78, 97% and 0.95, and 70% and 0.44, respectively. The cELISA method may be a technically more reproducible, objective, and convenient approach for piroplasmosis, dourine, and glanders serodiagnosis in qualifying animals for international movement and disease eradication programs than the CF systems currently in use. Use of the cELISA method also obviated the problems associated with testing hemolyzed or anticomplementary sera.
开发了程序相似的竞争性酶联免疫吸附测定（cELISA）方法，用于马巴贝斯虫属的马巴贝斯虫和驽巴贝斯虫（梨形虫病）、马媾疫锥虫（媾疫）以及鼻疽伯克霍尔德菌（鼻疽）感染的血清学诊断。马巴贝斯虫、驽巴贝斯虫、马媾疫锥虫和鼻疽伯克霍尔德菌cELISA的表观检测特异性分别为99.2%、99.5%、98.9%和98.9%。在实验感染的马中，用于马巴贝斯虫、驽巴贝斯虫、马媾疫锥虫和鼻疽伯克霍尔德菌感染血清学诊断的补体结合（CF）和cELISA程序之间的一致性和kappa值分别为76%和0.55、89%和0.78、97%和0.95以及70%和0.44。对于符合国际运输和疾病根除计划条件的动物，cELISA方法在梨形虫病、媾疫和鼻疽的血清学诊断中可能是一种技术上更具可重复性、客观性和便利性的方法，优于目前使用的CF系统。使用cELISA方法还避免了与检测溶血或抗补体血清相关的问题。
