Quirke J M, Hsu Y L, Van Berkel G J
Department of Chemistry, Florida International University, Miami, Florida 33199, USA.
J Nat Prod. 2000 Feb;63(2):230-7. doi: 10.1021/np990407l.
The alcohols and phenols of oil of cloves, lemon oil, rose absolut, and oil of peppermint were derivatized with ferroceneoyl azide to generate their ferroceneoyl carbamates. These derivatives are selectively detected at the attomole level, in nanomolar concentrations by electrospray-tandem mass spectrometry (ES-MS/MS) without the need for sample cleanup. The ES-MS/MS analyses of the four essential oils revealed all the expected alcohols, and, in the case of lemon oil, it detected alpha-terpineol as a trace component that was not readily observed by GC-MS. The ES-MS/MS analyses complements the more conventional GC-MS analysis. The ES-MS method has the advantage of speed, selectivity, and sensitivity over GC-MS for detection of a targeted alcohol of a specific mass or structural type. The ES-MS method does not require a chromatographic separation of the components to accomplish its task. In contrast, GC-MS remains the preferred method for the determination of the total constituents of an oil. The ES-MS method may produce artifact ions, especially if the sample is wet and an excess of the ferroceneoyl azide is used; however, the artifacts did not interfere with the analyses.
丁香精油、柠檬精油、纯玫瑰精油和薄荷精油中的醇类和酚类与二茂铁酰叠氮化物衍生化，生成它们的二茂铁酰氨基甲酸酯。通过电喷雾串联质谱法（ES-MS/MS），这些衍生物在阿托摩尔水平上就能被选择性检测，在纳摩尔浓度下无需样品净化。对这四种精油的ES-MS/MS分析揭示了所有预期的醇类，就柠檬精油而言，它检测到α-松油醇作为一种痕量成分，而气相色谱-质谱联用（GC-MS）不易观察到该成分。ES-MS/MS分析补充了更为传统的GC-MS分析。对于检测特定质量或结构类型的目标醇类，ES-MS方法在速度、选择性和灵敏度方面优于GC-MS。ES-MS方法不需要对成分进行色谱分离就能完成其任务。相比之下，GC-MS仍然是测定精油总成分的首选方法。ES-MS方法可能会产生伪离子，特别是如果样品潮湿且使用过量的二茂铁酰叠氮化物时；然而，这些伪离子并不干扰分析。
