心房利钠肽对豚鼠过敏反应后普萘洛尔诱导的支气管收缩的支气管保护作用。
Bronchoprotective effects of atrial natriuretic peptide against propranolol-induced bronchoconstriction after allergic reaction in guinea pigs.
作者信息
Mizuguchi M, Myo S, Fujimura M
机构信息
The Third Department of Internal Medicine, Kanazawa University School of Medicine, Kanazawa, Japan.
出版信息
Clin Exp Allergy. 2000 Mar;30(3):439-44. doi: 10.1046/j.1365-2222.2000.00725.x.
OBJECTIVE
To study the effects of intravenous atrial natriuretic peptide (ANP) on antigen-induced bronchoconstriction, propranolol-induced bronchoconstriction (PIB) after antigen challenge, and histamine-induced bronchoconstriction in guinea pigs.
METHODS
Allergic bronchoconstriction was evoked by inhalation of ovalbumin (OA) and PIB was caused when 10 mg/mL of propranolol was inhaled 20 min after OA challenge in passively sensitized and artificially ventilated guinea pigs. 25, 50, 100 and 200 microg/mL of histamine were inhaled for 20 s at 5-min intervals in non-sensitized guinea pigs.
RESULTS
Pretreatment with ANP in doses of 0.1 and 1.0 nmol/kg injected intravenously 15 min after antigen challenge reduced PIB in a dose-dependent manner, and 5 min before antigen challenge significantly attenuated PIB but not antigen-induced bronchoconstriction. Intravenous ANP significantly reduced bronchial responses to increasing concentrations of inhaled histamine in a dose-dependent manner.
CONCLUSION
These results suggest that ANP possesses protective effects against propranolol-induced and histamine-induced bronchoconstriction, albeit by a non-specific mechanism in guinea pig in vivo.
目的
研究静脉注射心房利钠肽（ANP）对豚鼠抗原诱导的支气管收缩、抗原激发后普萘洛尔诱导的支气管收缩（PIB）以及组胺诱导的支气管收缩的影响。
方法
在被动致敏并人工通气的豚鼠中，通过吸入卵清蛋白（OA）诱发过敏性支气管收缩，在OA激发20分钟后吸入10mg/mL普萘洛尔引发PIB。在未致敏的豚鼠中，以5分钟的间隔吸入25、50、100和200μg/mL组胺，持续20秒。
结果
抗原激发15分钟后静脉注射剂量为0.1和1.0nmol/kg的ANP进行预处理，可剂量依赖性地减轻PIB，在抗原激发前5分钟显著减弱PIB，但不影响抗原诱导的支气管收缩。静脉注射ANP可剂量依赖性地显著降低支气管对递增浓度吸入组胺的反应。
结论
这些结果表明，ANP对普萘洛尔诱导和组胺诱导的支气管收缩具有保护作用，尽管在豚鼠体内是通过非特异性机制实现的。
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