Franco J M, León-Leal J A, Leal M, Cano-Rodriguez A, Pineda J A, Macías J, Rubio A, Rey C, Sanchez B, Lissen E
Viral Hepatitis and AIDS Study Group, and Departments of Paediatrics, Radiology, Biochemistry and Immunology, Virgen del Rocío University Hospital, Seville, Spain.
Clin Exp Immunol. 2000 Mar;119(3):493-8. doi: 10.1046/j.1365-2249.2000.01152.x.
Previous studies have shown a slow recovery of naive CD4+ T cell counts after anti-retroviral therapy in HIV-1-infected adults, which is in accordance with thymus atrophy after puberty. Here we investigate whether or not different patterns of naive CD4+ and CD8+ T cell repopulation are present in adult and child patients undergoing anti-retroviral treatment. Thus, 25 adults under highly active anti-retroviral therapy and 10 children under combined anti-retroviral therapy were retrospectively analysed for T cell subpopulations at baseline (T0) and around week 12 (T1) and week 24 (T2) of anti-retroviral treatment. Mean serum HIV-1 RNA levels dropped in both groups. Recovery of T cells in adults was characterized by a heterogeneous response between patients, with only 44% of them increasing their naive CD4+ and CD8+ T cell counts at T1, and changes in mean total CD4+ T cells were mainly shaped by memory cells. Otherwise, children were characterized by an early increase in naive T cells. Thus, at T1, all children analysed had a strong rise in CD4+ (from 389 +/- 116 to 569 +/- 121 cells/microl; P < 0.01), and nine out of 10 also in naive CD8+ T cells (from 244 +/- 58 to 473 +/- 85 cells/microl; P < 0.05). However, no significant correlation between age and naive repopulation was observed (P = 0. 22) in children. Thus, children had the earlier and greater increases in naive T cell subsets than adults, probably due to a more active thymus, with the potential for immune reconstitution when HIV-1 replication is controlled.
先前的研究表明，在接受抗逆转录病毒治疗后，HIV-1感染的成年人体内初始CD4+T细胞计数恢复缓慢，这与青春期后胸腺萎缩一致。在此，我们调查接受抗逆转录病毒治疗的成年和儿童患者中，初始CD4+和CD8+T细胞再增殖模式是否存在差异。因此，我们对25名接受高效抗逆转录病毒治疗的成年人和10名接受联合抗逆转录病毒治疗的儿童进行了回顾性分析，检测他们在抗逆转录病毒治疗基线期（T0）、治疗约12周（T1）和24周（T2）时的T细胞亚群。两组患者的平均血清HIV-1 RNA水平均下降。成人T细胞的恢复表现为患者之间的异质性反应，只有44%的患者在T1时初始CD4+和CD8+T细胞计数增加，平均总CD4+T细胞的变化主要由记忆细胞决定。相比之下，儿童的特点是初始T细胞早期增加。因此，在T1时，所有接受分析的儿童CD4+T细胞均显著增加（从389±116个细胞/微升增至569±121个细胞/微升；P<0.01），10名儿童中有9名的初始CD8+T细胞也增加（从244±58个细胞/微升增至473±85个细胞/微升；P<0.05）。然而，在儿童中未观察到年龄与初始再增殖之间存在显著相关性（P = 0.22）。因此，儿童初始T细胞亚群的增加比成年人更早、幅度更大，这可能是由于胸腺更活跃，在HIV-1复制得到控制时具有免疫重建的潜力。
