Amoresano A, Amedeo S, D'andrea G, Siciliano R, Gagna C, Castagnaro M, Marino G, Guarda F
Centro Internazionale di Servizi di Spettrometria di Massa, CNR-Università, Napoli, Italy.
Eur J Biochem. 2000 Mar;267(5):1299-306. doi: 10.1046/j.1432-1327.2000.01090.x.
Endocardiosis, a degenerative and dystrophic process affecting cardiac valves and described in many mammalian species, is characterized by the accumulation of glycosaminoglycans, in particular hyaluronic acid, in the extracellular matrix. The glycoprotein patterns of pig mitral valves in normal animals and animals affected by endocardiosis were investigated. A different N-linked glycosylation pattern of glycoproteins was detected in affected valves compared with normal ones. In either normal or pathological species, the detected N-linked glycans were of the complex type. However, in samples from affected valves, sialic acid showed a prevalence of the alpha2,6 linkage to the galactosyl residue, whereas in normal samples the most frequent linkage was of the alpha2,3 type. In normal valves, the majority of complex oligosaccharides presented two outer branches with different degrees of fucosylation and sialylation, whereas in pathological samples we noted an increased number of glycans having up to four outer branches.
心内膜变性，是一种影响心脏瓣膜的退行性和营养不良性过程，在许多哺乳动物物种中都有描述，其特征是糖胺聚糖，特别是透明质酸，在细胞外基质中积累。研究了正常动物和患心内膜变性动物的猪二尖瓣糖蛋白模式。与正常瓣膜相比，在患病瓣膜中检测到了不同的糖蛋白N-连接糖基化模式。在正常或患病物种中，检测到的N-连接聚糖均为复合型。然而，在患病瓣膜的样本中，唾液酸与半乳糖残基的α2,6连接占优势，而在正常样本中最常见的连接是α2,3型。在正常瓣膜中，大多数复合寡糖呈现出两个具有不同程度岩藻糖基化和唾液酸化的外分支，而在病理样本中，我们注意到具有多达四个外分支的聚糖数量增加。
