Zielke D R, Heggers J P, Harrison J W
Oral Surg Oral Med Oral Pathol. 1976 Dec;42(6):830-7. doi: 10.1016/0030-4220(76)90107-9.
Asymptomatic teeth were cultured at four specific stages of endodontic therapy, yielding 148 paired samples. Half of the samples were incubated anaerobically with the use of a new medium, and half were incubated aerobically with the use of a commonly employed endodontic medium. The former proved to be significantly more sensitive in supporting microbial growth from root canals. The results indicate that an aerobic culturing technique alone is not sufficient to reflect the microbiologic status of the canal system.
在牙髓治疗的四个特定阶段对无症状牙齿进行培养，得到148对样本。一半样本使用一种新培养基进行厌氧培养，另一半样本使用常用的牙髓培养基进行需氧培养。结果表明，前者在支持根管内微生物生长方面明显更敏感。结果表明，仅需氧培养技术不足以反映根管系统的微生物学状况。
