Malone B R, Humphrey C W, Romer T R, Richard J L
Romer Labs, Inc., Union, MO 63084-1156, USA.
J AOAC Int. 2000 Jan-Feb;83(1):95-8.
A rapid, quantitative, inexpensive, and efficient method was developed to determine aflatoxins in corn, corn meal, popcorn, rice, wheat, cottonseed, and peanuts. Samples are ground and extracted with methanol-water (80 + 20). A portion of the extract is cleaned up by passage through a solid-phase separatory column, 500 microL purified extract is derivatized with a bromine reagent, and fluorescence of the solution is immediately quantified with a calibrated fluorometer containing a broad wavelength pulsed xenon light source. This method can quantify aflatoxin from 5 to 5000 ppb without dilution and was linear when applied to samples of noncontaminated corn spiked at 0 to 5000 micrograms aflatoxin B1/g. Correlation coefficients of the method with LC for multiple analyses for corn (n = 34), cottonseed (n = 32), and peanuts (n = 11) were 0.999, 0.995, and 0.980, respectively. Individual analyses may be conducted in less than 5 min, and grouping of samples is unnecessary. The sensitivity of the method for corn is 5 ppb and the fluorometer, under the operating conditions, has a limit of detection of 0.6 ng aflatoxin B1.
开发了一种快速、定量、廉价且高效的方法来测定玉米、玉米粉、爆米花、大米、小麦、棉籽和花生中的黄曲霉毒素。将样品研磨后用甲醇 - 水（80 + 20）提取。提取液的一部分通过固相分离柱进行净化，500微升纯化后的提取液用溴试剂衍生化，然后立即用配有宽波长脉冲氙光源的校准荧光计对溶液的荧光进行定量。该方法无需稀释即可对5至5000 ppb的黄曲霉毒素进行定量，应用于添加了0至5000微克黄曲霉毒素B1/克的未受污染玉米样品时呈线性关系。该方法与液相色谱法对玉米（n = 34）、棉籽（n = 32）和花生（n = 11）进行多次分析的相关系数分别为0.999、0.995和0.980。单次分析可在不到5分钟内完成，无需对样品进行分组。该方法对玉米的灵敏度为5 ppb，在操作条件下，荧光计对黄曲霉毒素B1 的检测限为0.6纳克。
