Menkissoglu-Spiroudi U, Diamantidis G C, Georgiou V E, Thrasyvoulou A T
Aristotle University of Thessaloniki, Department of Agriculture, Greece.
J AOAC Int. 2000 Jan-Feb;83(1):178-82.
A rapid, reliable, and inexpensive extraction method was developed to determine acaricide residues in honey by gas chromatography (GC) with nitrogen-phosphorus (NP) or electron capture (EC) detectors. Because of the high selectivity of the NP detector, no interfering peaks were present and no cleanup was necessary. A simple cleanup step is proposed for the GC-ECD analysis. Recoveries from spiked honey samples ranged from 79 to 94.4%, with coefficients of variation of 0.3-18.5%. The quantitation limit obtained was 0.015 mg/kg for malathion, 0.020 mg/kg for coumaphos, and 0.005 mg/kg for fluvalinate. The method was used to determine the disappearance of malathion and coumaphos residues from honey samples collected from beehives treated with these acaricides. The disappearance of both acaricides was rapid and followed a first-order model for the duration of the experiment.
开发了一种快速、可靠且廉价的提取方法，用于通过配备氮磷（NP）或电子捕获（EC）检测器的气相色谱法（GC）测定蜂蜜中的杀螨剂残留。由于NP检测器具有高选择性，不存在干扰峰，无需净化。针对GC-ECD分析提出了一个简单的净化步骤。加标蜂蜜样品的回收率在79%至94.4%之间，变异系数为0.3%至18.5%。所获得的定量限为：马拉硫磷0.015 mg/kg，蝇毒磷0.020 mg/kg，氟氯苯菊酯0.005 mg/kg。该方法用于测定从用这些杀螨剂处理过的蜂箱中采集的蜂蜜样品中马拉硫磷和蝇毒磷残留的消失情况。在实验期间，两种杀螨剂的消失速度都很快，且符合一级模型。
