Gaĭnutdinova T Kh, Andrianov V V, Nazyrova R R, Gaĭnutdinov Kh L
Group of Biophysics, Kazan Physico-Technological Institute, Russian Academy of Sciences.
Zh Vyssh Nerv Deiat Im I P Pavlova. 1999 Nov-Dec;49(6):1063-5.
The retention of the long-term sensitization (LTS) of defensive reflex and dynamics of change in electric characteristics (membrane potential (Vm) and action potential generation threshold (Vt)) of command neurons of defensive reflex was studied in a snail during behavioral tests. The membrane mechanisms were analyzed by measuring electrical characteristics of the LPa3, RPa3, LPa2, and RPa2 command neurons on the 1st, 4th, 7th, 10th, and 14th days after the LTS formation and 1 month later. The membrane potential and threshold potential in sensitized snails (-54.1 +/- 2.0 and 24.5 +/- 1.4 microV, respectively) were significantly (p < 0.001) decreased in comparison with the control animals (-60.9 +/- 0.8 and 19.9 +/- 0.6 microV respectively). These changes retained within 14 days after the LTS formation. The results suggest the long-term retention of the increased excitability of command neurons. A month after the LTS formation, the duration of the defensive reflex returned to the initial level and the electric characteristics of command neurons did not significantly differ from the control (-61.1 +/- 2.0 and 19.3 +/- 1.4 microV, respectively).
在行为测试期间，研究了蜗牛防御反射的长期敏感化（LTS）的维持以及防御反射指令神经元电特性（膜电位（Vm）和动作电位产生阈值（Vt））的变化动态。通过在LTS形成后的第1天、第4天、第7天、第10天和第14天以及1个月后测量LPa3、RPa3、LPa2和RPa2指令神经元的电特性来分析膜机制。与对照动物（分别为-60.9±0.8和19.9±0.6 μV）相比，敏感化蜗牛的膜电位和阈值电位（分别为-54.1±2.0和24.5±1.4 μV）显著降低（p<0.001）。这些变化在LTS形成后的14天内持续存在。结果表明指令神经元兴奋性增加的长期维持。在LTS形成1个月后，防御反射的持续时间恢复到初始水平，指令神经元的电特性与对照无显著差异（分别为-61.1±2.0和19.3±1.4 μV）。
