Strunz U, Domschke W, Domschke S, Mitznegg P, Wünsch E, Jaeger E, Demling L
Scand J Gastroenterol Suppl. 1976;39:29-33.
The interaction of 13-nle-motilin-a synthetic analogue of the natural polypeptide-with acetylcholine was studied on rabbit isolated pyloric sphincter in vitro. Adding subthreshold doses of 13-nle-motilin reduced the calculated acetylcholine dose for half maximal contraction from 3.68 to 1.33 X 10(-6) g/ml and increased the calculated maximal response by 15.3%. Thus 13-nle-motilin caused potentiation of the acetylcholine response. Since 13-nle-motilin induced increase of endogenous acetylcholine levels could be excluded, a direct action of motilin on or in the smooth muscle cell is suggested. The results are compatible with the view that in vivo subthreshold doses of motilin may affect cholinergic driven pyloric motor activity.
在体外对兔离体幽门括约肌研究了天然多肽的合成类似物13-去甲亮氨酸-胃动素与乙酰胆碱的相互作用。添加阈下剂量的13-去甲亮氨酸-胃动素可使计算得出的产生半数最大收缩的乙酰胆碱剂量从3.68降至1.33×10⁻⁶ g/ml，并使计算得出的最大反应增加15.3%。因此，13-去甲亮氨酸-胃动素引起了乙酰胆碱反应的增强。由于可以排除13-去甲亮氨酸-胃动素诱导内源性乙酰胆碱水平升高的情况，提示胃动素对平滑肌细胞有直接作用或在平滑肌细胞内有直接作用。这些结果与以下观点相符，即体内阈下剂量的胃动素可能影响胆碱能驱动的幽门运动活性。
