Christensen S K, Dagenais N, Chory J, Weigel D
The Salk Institute for Biological Studies, La Jolla, California 92037, USA.
Cell. 2000 Feb 18;100(4):469-78. doi: 10.1016/s0092-8674(00)80682-0.
Arabidopsis plants carrying mutations in the PINOID (PID) gene have a pleiotropic shoot phenotype that mimics that of plants grown on auxin transport inhibitors or of plants mutant for the auxin efflux carrier PINFORMED (PIN), with defects in the formation of cotyledons, flowers, and floral organs. We have cloned PID and find that it is transiently expressed in the embryo and in initiating floral anlagen, demonstrating a specific role for PID in promoting primordium development. Constitutive expression of PID causes a phenotype in both shoots and roots that is similar to that of auxin-insensitive plants, implying that PID, which encodes a serine-threonine protein kinase, negatively regulates auxin signaling.
携带PID基因（PINOID）突变的拟南芥植株具有多效性茎表型，类似于在生长素运输抑制剂上生长的植物或生长素外排载体PIN形成（PIN）突变的植物，在子叶、花和花器官的形成上存在缺陷。我们已经克隆了PID，发现它在胚胎和起始花原基中短暂表达，表明PID在促进原基发育中具有特定作用。PID的组成型表达在茎和根中都引起一种类似于生长素不敏感植物的表型，这意味着编码丝氨酸 - 苏氨酸蛋白激酶的PID负调控生长素信号传导。
