Piqueras A, Olmos E, Martínez-Solano J R, Hellín E
Departamento de Nutrición y Fisiología Vegetal, Centro de Edafología y Biología Aplicada del Segura (CSIC) Murcia, Spain.
Free Radic Res. 1999 Dec;31 Suppl:S33-8. doi: 10.1080/10715769900301291.
The relation between Cd and oxidative stress in BY2 cell cultures of tobacco was studied. In response to 5 mM Cd, a rapid generation of H2O2 has been detected in tobacco cell cultures by the oxidative quenching of the fluorescent reporter dye pyranine. This oxidative burst reached the maximum production of H2O2 after 10 min of treatment with Cd. This response could be considered as short term hypersensitive response previous to the oxidative stress caused by the metal at the cell plasma membrane. The observed antioxidant enzymatic response to the oxidative burst was preceded by an increased peroxidation of lipids with a significant increase in the activities of superoxide dismutase and ascorbate peroxidase. The results presented in this study point out to the plasma membrane as the primary target for the short term production of activated oxygen species in response to Cd in BY2 tobacco cells followed by a coordinated activation of the antioxidant enzymatic system.
研究了烟草BY2细胞培养物中镉（Cd）与氧化应激之间的关系。响应5 mM的Cd，通过荧光报告染料吡喃荧光的氧化猝灭，在烟草细胞培养物中检测到过氧化氢（H2O2）的快速生成。在用Cd处理10分钟后，这种氧化爆发达到了H2O2的最大产量。这种反应可被视为在金属引起的细胞膜氧化应激之前的短期超敏反应。在观察到对抗氧化爆发的抗氧化酶反应之前，脂质过氧化增加，超氧化物歧化酶和抗坏血酸过氧化物酶的活性显著增加。本研究结果指出，细胞膜是BY2烟草细胞中响应Cd而短期产生活性氧的主要靶点，随后抗氧化酶系统会协同激活。
