al-Rawi N, Kavanagh K
Department of Biology, National University of Ireland, Maynooth, Ireland.
Br J Biomed Sci. 1999;56(2):99-104.
High-vaginal swabs were taken from 98 women attending a genitourinary clinic in Dublin city who presented with symptoms of vaginitis. Twenty-eight (28.6%) proved culture-positive for yeasts. Candida albicans was isolated from 26 of the yeast-positive patients and Candida glabrata and Candida tropicalis were isolated from a further two patients. Two patients were colonized by two yeasts simultaneously (C. albicans and Candida lucitaniae, and C. albicans and Candida krusei). Yeasts were characterised on the basis of their adherence ability, extracellular enzyme production and resistance to antifungal agents. All C. albicans isolates demonstrated high adherence abilities to buccal epithelial cells, but produced relatively low levels of phospholipase and acid proteinase. The non-C. albicans isolates demonstrated low levels of adherence, but no extracellular enzyme production was detected. Isolates were most sensitive to the polyenes amphotericin B and nystatin but a large proportion (50%) of C. albicans isolates were resistant to clotrimazole, which is an important agent in the treatment of vulvovaginal candidosis.
从都柏林市一家泌尿生殖科诊所就诊的98名出现阴道炎症状的女性中采集了高阴道拭子。28名（28.6%）被证明酵母培养呈阳性。26名酵母阳性患者分离出白色念珠菌，另外两名患者分离出光滑念珠菌和热带念珠菌。两名患者同时被两种酵母定植（白色念珠菌和葡萄牙念珠菌，以及白色念珠菌和克柔念珠菌）。根据酵母的黏附能力、细胞外酶产生情况和对抗真菌药物的耐药性对其进行了鉴定。所有白色念珠菌分离株对颊上皮细胞均表现出高黏附能力，但磷脂酶和酸性蛋白酶产生水平相对较低。非白色念珠菌分离株黏附水平较低，但未检测到细胞外酶产生。分离株对多烯类两性霉素B和制霉菌素最敏感，但很大比例（50%）的白色念珠菌分离株对克霉唑耐药，克霉唑是治疗外阴阴道念珠菌病的重要药物。
